人精囊上皮细胞-细胞株/菌种-试剂-生物在线
人精囊上皮细胞

人精囊上皮细胞

商家询价

产品名称: 人精囊上皮细胞

英文名称: Human Seminal Vesicle Epithelial Cells

产品编号: XY4460

产品价格: 0

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: null

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
  • 邮箱 : shxysw02@163.com

人精囊上皮细胞Cell Specification
The seminal vesicles (SV) are a pair of tubular glands located near the prostate and are essential
to the urinary system. They function under androgen control to produce and secrete fluid into the
ejaculatory duct. The SV consists of three layers: the inner basal mucosal layer composed of
simple cuboidal and pseudo-stratified columnar epithelial cells, the middle muscular layer
formed by smooth muscle cells, and the outer layer made up of dense connective tissue. Various
pathological conditions can arise in the SV, including congenital SV cysts, seminal vesculitis,
and primary and secondary neoplasms [1-3]. Additionally, SV invasion is often used as a
prognostic marker in prostate cancer [4]. SV epithelial cells offer unique opportunities to study
many features of the SV.
HSVEpiC from ScienCell Research Laboratories are isolated from human seminal vesicle tissue.
HSVEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105
cells in 1 ml volume. HSVEpiC are characterized by immunofluorescence with antibody specific
to cytokeratin (CK-18). HSVEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria,
yeast and fungi. HSVEpiC are guaranteed to further expand for 15 population doublings under
the conditions provided by ScienCell Research Laboratories.
人精囊上皮细胞Recommended Medium
It is recommended to use Epithelial Cell Medium-2 (EpiCM-2, Cat. #4121) for the culturing of
HSVEpiC in vitro.
Product Use
HSVEpiC are for research use only. It is not approved for human or animal use, or for
application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and
keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
References
[1] Arora SS, Breiman RS, Webb EM, Westphalen AC, Yeh BM, Coakley FV. (2007) “CT and MRI of congenital
anomalies of the seminal vesicles.” AJR Am J Roentgenol. 189: 130-5.
[2] Campobasso D, Fornia S, Ferretti S, Maestroni U, Cortellini P. (2012) “Primary bilateral seminal vesicle
carcinoma: description of a case and literature review.” Int J Surg Pathol. 20: 633-5.
[3] Wang J, Yue X, Zhao R, Cheng B, Wazir R, Wang K. (2013) “Primary squamous cell carcinoma of seminal
vesicle: an extremely rare case report with literature review.” Int Urol Nephrol. 45: 135-8.
[4] Kristiansen A, Wiklund F, Wiklund P, Egevad L. (2013) “Prognostic significance of patterns of seminal vesicle
invasion in prostate cancer.” Histopathology. 62: 1049-56.
Instructions for culturing cells
Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37oC water bath
and return the cells to culture as quickly as possible with minimal handling!
Initiating the culture:
1. Prepare a poly-L-lysine coated culture vessel (2 μg/cm2
, T-75 flask is recommended).
Add 10 ml of sterile water to a T-75 flask and then add 15 μl of poly-L-lysine stock
solution (10 mg/ml, Cat. #0413). Leave the vessel in incubator overnight (minimum one
hour at 37oC incubator).
2. Prepare complete medium. Decontaminate the external surfaces of medium bottle and
medium supplements with 70% ethanol and transfer them to sterile field. Aseptically
transfer supplement to the basal medium with a pipette. Rinse the tube with medium to
recover the entire volume.
3. Rinse the poly-L-lysine coated vessel with sterile water twice and then add 15 ml of
complete medium. Leave the vessel in the sterile field and proceed to thaw the
cryopreserved cells.
4. Place the frozen vial in a 37oC water bath. Hold and rotate the vial gently until the
contents completely thaw. Remove the vial from the water bath promptly, wipe it down
with 70% ethanol and transfer it to a sterile field.
5. Remove the cap carefully without touching the interior threads. Gently resuspend and
人精囊上皮细胞dispense the contents of the vial into the equilibrated, poly-L-lysine coated culture vessel.
A seeding density of 5,000 cells/cm2
is recommended.
Note: Dilution and centrifugation of cells after thawing are not recommended since these
actions are more harmful to the cells than the effect of residual DMSO in the culture. It
is also important that cells are plated in poly lysine coated culture vessels to promote
cell attachment.
6. Replace the cap or lid, and gently rock the vessel to distribute the cells evenly. Loosen
cap if necessary to allow gas exchange.
7. Return the culture vessel to the incubator.
8. For the best result, do not disturb the culture for at least 16 hours after the culture has
been initiated. Refresh culture medium the next day to remove the residual DMSO and
unattached cells, then every other day thereafter.
Maintaining the culture:
1. Refresh supplemented culture medium the next morning after establishing a culture from
cryopreserved cells.
2. Change the medium every three days thereafter, until the culture is approximately 70%
confluent.
3. Once the culture reaches 70% confluency, change medium every other day until the
culture is approximately 90% confluent.
Subculturing:
1. Subculture when the culture reaches 90% confluency or above.
2. Prepare poly-L-lysine-coated culture vessels (2 μg/cm2
) one day before subculture.
3. Warm complete medium, trypsin/EDTA solution (T/E, Cat. #0103), T/E neutralization
solution (TNS, Cat. #0113), and DPBS (Ca++
- and Mg++
-free, Cat. #0303) to room
temperature. We do not recommend warming reagents and medium in a 37oC water bath
prior to use.
4. Rinse the cells with DPBS.
5. Add 8 ml of DPBS and then 2 ml of T/E solution into flask (in the case of a T-75 flask).
Gently rock the flask to ensure complete coverage of cells by T/E solution. Incubate the
flask in a 37oC incubator for 1 to 2 minutes or until cells completely round up. Use a
microscope to monitor the change in cell morphology.
6. During incubation, prepare a 50 ml conical centrifuge tube with 5 ml of fetal bovine
serum (FBS, Cat. #0500).
7. Transfer T/E solution from the flask to the 50 ml centrifuge tube (a small percent of cells
may detach) and continue to incubate the flask at 37oC for another 1 to 2 minutes (no
solution in the flask at this moment).
8. At the end of incubation, gently tap the side of the flask to dislodge cells from the
surface. Check under a microscope to make sure that all cells detach.
人精囊上皮细胞9. Add 5 ml of TNS solution to the flask and transfer detached cells to the 50 ml centrifuge
tube. Rinse the flask with another 5 ml of TNS to collect the residual cells.
10. Examine the flask under a microscope for a successful cell harvest by looking at the
number of cells being left behind; there should be less than 5%.
Note: Use ScienCell T/E solution that is optimized to minimize cell damages due to over
trypsinization.
11. Centrifuge the 50 ml centrifuge tube at 1000 rpm for 5 minutes. Resuspend cells in
culture medium.
12. Count and plate cells in a new poly-L-lysine-coated culture vessel with the recommended
cell density.
Caution: Handling human derived products is potentially biohazardous. Although each cell
strain tests negative for HIV, HBV and HCV DNA, diagnostic tests are not necessarily 100%
accurate, therefore, proper precautions must be taken to avoid inadvertent exposure. Always
wear gloves and safety glasses when working with these materials. Never mouth pipette. We
人精囊上皮细胞recommend following the universal procedures for handling products of human origin as the
minimum precaution against contamination [1].
[1] Grizzle WE, Polt S. (1988) “Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues.” J Tissue Culture Methods. 11: 191-9.