人主动脉平滑肌细胞-细胞株/菌种-试剂-生物在线
人主动脉平滑肌细胞

人主动脉平滑肌细胞

商家询价

产品名称: 人主动脉平滑肌细胞

英文名称: Human Aortic Smooth Muscle Cells

产品编号: XY6110

产品价格: 0

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: null

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
  • 邮箱 : shxysw02@163.com

人主动脉平滑肌细胞Cell Specification
Smooth muscle cell (SMC) is the cellular substate of most significant arterial disease [1]. The
increased growth potential of vascular SMC represents one of the crucial anomalies responsible
for the development of essential vascular diseases. New studies demonstrate that SMC express
calcium channels [2] and the expression of ICAM-1 and VCAM-1 on SMC may contribute to the
inflammatory reaction in the vascular wall and may actively be involved in the progression and
stability of vascular disease [3]. In vitro culture of human vascular SMC as a model of vascular
research played a critical role and continues providing information in the pharmacology and the
therapy of vascular diseases.
HASMC from ScienCell Research Laboratories are isolated from human aorta. HASMC are
cryopreserved at secondary culture after purification and delivered frozen. Each vial contains >5
x 105
cells in 1 ml volume. HASMC are characterized by immunofluorescent method with
antibodies to α-smooth muscle actin and desmin. HASMC are negative for HIV-1, HBV, HCV,
mycoplasma, bacteria, yeast and fungi. HASMC are guaranteed to further expand for 15
population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. No. 1101) for the culturing
of HASMC in vitro.
人主动脉平滑肌细胞Product Use
HASMC are for research use only. It is not approved for human or animal use, or for application
in in vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep
the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Schwartz, S. M., Campbell, G. R., Campbell, J. H. (1986) Replication of smooth muscle cells in vascular
disease. Circ. Res. 58:427-444.
[2] Fan, Q. I., Vanderpool, K., Marsh, J. D. (2002) A 27 bp cis-acting sequence is essential for L-type calcium
channel alpha(1C) subunit expression in vascular smooth muscle cells. Biochim Biophys Acta. 1577:401-11.
[3] Braun, M., Pietsch, P., Schror, K., Baumann, G., Felix, S. B. (1999) Cellular adhesion molecules on vascular
smooth muscle cells. Cardiovasc. Res. 41:395-401.
Instruction for culturing cells
Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37o
C waterbath
and return them to culture as quickly as possible with minimal handling!
Set up culture after receiving the ordering:
1. Place the vial in a 37o
C waterbath, hold and rotate the vial gently until the contents are
completely thawed. Remove the vial from the waterbath immediately, wipe it dry, and
transfer it to a sterile field. Rinse the vial with 70% ethanol, and then wipe to remove
excess. Remove the cap, being careful not to touch the interior threads with fingers.
Using 1 ml eppendorf pipette gently resuspend the contents of the vial.
人主动脉平滑肌细胞2. Dispense the contents of the vial into the equilibrated, poly-L-lysine coated culture
vessels. A seeding density of 7,500 cells/cm2
is recommended.
Note: Dilution and centrifugation of cells after thawing are not recommended since these
actions are more harmful to the cells than the effect of DMSO residue in the culture. It is
also important that HASMC are plated in poly-L-lysine coated culture vessels that
promote vascular smooth muscle cell attachment.
3. Replace the cap or cover, and gently rock the vessel to distribute the cells evenly. Loosen
caps if necessary to permit gas exchange.
4. Return the culture vessels to the incubator.
5. For best result, do not disturb the culture for at least 16 hours after the culture has been
initiated. Change the growth medium the next day to remove the residual DMSO and
unattached cells, then every other day thereafter. A health culture will display spindle
shaped, usually in a homogeneous bundle or sheet of cells rather than scattered single
cells and the cell number will be doubled after two to three days in culture.
Maintenance of Culture:
1. Change the medium to fresh supplemented medium the next morning after establishing a
culture from cryopreserved cells. For subsequent subcultures, change medium 48 hours
after establishing the subculture.
2. Change the medium every other day thereafter, until the culture is approximately 50%
confluent.
3. Once the culture reaches 50% confluence, change medium every day until the culture is
approximately 80% confluent.
Subculture:
1. Subculture the cells when they are 80% confluent.
2. Prepare poly-L-lysine coated cell culture flasks.
3. Warm medium, trypsin/EDTA solution, trypsin neutralization solution, and DPBS to
room temperature. We do not recommend warming the reagents and medium at 37o
C
waterbath prior to use.
4. Rinse the cells with DPBS.
5. Incubate cells with 10 ml of trypsin/EDTA solution (in the case of T-75 flask) until 80%
of cells are rounded up (monitored with microscope). Add 10 ml of trypsin neutralization
solution to the digestion immediately and gently rock the culture vessel.
Note: Use ScienCell Research Laboratories’ trypsin/EDTA solution that is optimized to
minimize the killing of the cells by over trypsinization.
6. Harvest and transfer released cells into a 50 ml centrifuge tube. Rinse the flask with
another 10 ml of growth medium to collect the residue cells. Examine the flask under
microscope to make sure the harvesting is successful by looking at the number of cells
left behind. There should be less than 5%.
7. Centrifuge the harvested cell suspension at 1000 rpm for 5 min and resuspend cells in
growth medium.
8. Count cells and plate them in a new, poly-L-lysine coated flask with cell density as
recommended.
Caution: Handling human derived products is potentially bioharzadous. Although each cell strain testes negative
for HIV, HBV and HCV DNA, diagnostic tests are not necessarily 100% accurate, therefore, proper precautions
mush be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working these materials.
人主动脉平滑肌细胞Never mouth pipette. We recommend following the universal procedures for handling products of human origin as
the minimum precaution against contamination [1].
[1]. Grizzle, W. E., and Polt, S. S. (1988) Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues. J Tissue Culture Methods. 11(4).