小鼠肝巨噬细胞-细胞株/菌种-试剂-生物在线
小鼠肝巨噬细胞

小鼠肝巨噬细胞

商家询价

产品名称: 小鼠肝巨噬细胞

英文名称: MHMa

产品编号: XYM5340-57

产品价格: 0

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: null

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
  • 邮箱 : shxysw02@163.com

 小鼠肝巨噬细胞Cell Specification
Macrophages are cells differentiated from circulating bone marrow-derived monocytes. The
main function of macrophages is to remove cellular debris and destroy invading pathogens.
Mouse Hepatic Macrophages (MHMa), which are also known as Kupffer cells, reside within the
lumen of liver sinusoids. MHMa protect the liver by responding to pathogens and metastatic
cells, while tolerating harmless self and foreign antigens, which enter via blood flow through the
portal vein and hepatic artery [1]. Recent studies have shown that hepatic macrophages play an
important role in fibrosis, liver inflammation, fatty liver disease, and liver transplantation [2-4].
MHMa are an excellent model for studying macrophage functions under normal physiological
and pathological conditions.
MHMa from ScienCell Research Laboratories are isolated from postnatal day 2 mouse liver.
MHMa are cryopreserved after purification and delivered frozen. Each vial contains > 1 x 106
cells in 1 ml volume. MHMa are characterized by immunofluorescence with antibody to F4/80.
MHMa are negative for mycoplasma, bacteria, yeast, and fungi. MHMa are guaranteed to further
culture in the conditions provided by ScienCell Research Laboratories; however, HMa are not
recommended for expanding or long term cultures since the cells do not proliferate in regular
culture.
 小鼠肝巨噬细胞Recommended Medium
It is recommended to use Macrophage Medium (MaM, Cat. #1921) for culturing MHMa in vitro.
Product Use
MHMa are for research use only. They are not approved for human or animal use, or for
application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and
keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
References
[1] Liaskou E, Wilson DV, Oo YH. (2012) “Innate immune cells in liver inflammation.” Mediators Inflamm. 2012:
949157.
[2] Bieghs V, Verheyen F, van Gorp PJ, Hendrikx T, Wouters K, Lütjohann D, Gijbels MJ, Febbraio M, Binder CJ,
Hofker MH, Shiri-Sverdlov R. (2012) “Internalization of modified lipids by CD36 and SR-A leads to hepatic
inflammation and lysosomal cholesterol storage in Kupffer cells.” PLoS One. 7: e34378.
[3] Tian Y, Jochum W, Georgiev P, Moritz W, Graf R, Clavien PA. (2006) “Kupffer cell-dependent TNF-alpha
signaling mediates injury in the arterialized small-for-size liver transplantation in the mouse.” Proc Natl Acad Sci U
S A. 103: 4598-603.
[4] Seki E, de Minicis S, Inokuchi S, Taura K, Miyai K, van Rooijen N, Schwabe RF, Brenner DA. (2009) “CCR2
promotes hepatic fibrosis in mice.” Hepatology. 50: 185-97.
Instructions for culturing cells
 小鼠肝巨噬细胞Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37oC water bath
and return the cells to culture as quickly as possible with minimal handling!
Note: Experiments should be well organized before thawing MHMa. It is recommended that
MHMa are used for experiments as quickly as possible after thawing the cells. MHMa cannot be
subcultured or passaged as the cells do not proliferate.
Initiating the culture:
1. Prepare a poly-L-lysine-coated culture plate (2 μg/cm2
is recommended). For example, add 2
ml of sterile water to one well of a 6-well plate and then add 20l of poly-L-lysine stock
solution (1 mg/ml, Cat. #0403). Leave the plate in a 37oC incubator overnight (or for a
minimum of one hour).
2. Prepare complete medium (MaM, Cat. #1921). Thaw MaGS, FBS and P/S solution at 37oC.
Gently tilt the MaGS tube several times to ensure the contents are completely dissolved
before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and wipe to
remove excess ethanol. In a sterile field, remove the cap, being careful not to touch the
interior threads with fingers. Add MaGS, FBS and P/S solution to the medium and mix well.
3. Rinse the poly-L-lysine-coated vessel twice with sterile water and then add the volume of
complete medium recommended in Table 1 or Table 2. Leave the plate(s) in the sterile field
and proceed to thaw the cryopreserved cells.
4. Place the frozen vial in a 37oC water bath. Hold and rotate the vial gently until the contents
completely thaw. Promptly remove the vial from the water bath, wipe it down with 70%
ethanol, and transfer it to the sterile field.
Note: Dilution and centrifugation of cells after thawing are not recommended since these
actions are more harmful to the cells than the effect of residual DMSO in the culture. It is
also important that cells are plated in poly lysine coated culture vessels to promote cell
attachment.
 小鼠肝巨噬细胞5. Carefully remove the cap without touching the interior threads and gently resuspend the cell
suspension. A seeding density of 10,000-20,000 cells/cm2
is recommended depending on
your experiments. We recommend following Table 1 for seeding MHMa onto 6-well, 12-
well, or 24-well plates. For seeding MHMa on 60 mm plates, use Table 2.
Table 1
Recommended cell suspension volume per vial using a 6-well, 12-well, or 24 well format
Well format Surface area/well
(approx. values)
Volume of media/well Volume of cell suspension
from vial/well
# of wells/vial
6-well 9.6 cm2 3.0 ml 150 l 6 wells
12-well 3.9 cm2 2.0 ml 60 l 15 wells
24-well 1.9 cm2 1.0 ml 30 l 30 wells
Table 2
Recommended cell suspension volume per vial using 60 mm plates
Plate Format Surface area/plate
(approx. values)
Volume of cell
suspension from vial/plate
# of plates/vial Volume of media
(ml)/plate
60 mm 21 cm2
300 l 3 3.0 ml
6. Pipet the correct volume of cell suspension into each well of an equilibrated, poly-Llysine-coated
culture plate containing complete medium. Replace the lid of the culture
plate and gently rock the plate to distribute the cells evenly.
7. Return the culture plate to the incubator.
8. For best results, do not disturb the culture for at least 16 hours after the culture has been
initiated. Refresh the culture medium the next morning after establishing a culture from
cryopreserved cells to remove residual DMSO and unattached cells.
9. Use cells promptly for experiments.
Caution: Handling human derived products is potentially biohazardous. Although each cell strain tests
negative for HIV, HBV and HCV DNA, diagnostic tests are not necessarily 100% accurate, therefore,
 小鼠肝巨噬细胞proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses
when working with these materials. Never mouth pipette. We recommend following the universal
procedures for handling products of human origin as the minimum precaution against contamination [1].
[1] Grizzle WE, Polt S. (1988) “Guidelines to avoid personal contamination by infective agents in research
laboratories that use human tissues.” J Tissue Culture Methods. 11: 191-9.