大鼠淋巴单核细胞Cell Specification
Lymphatic Mononuclear Cells (LMC) are located in the lymph nodes and perform vital functions
for the innate and adaptive immune system [1]. Primary LMC are a mixed population of single
nucleus cells which include T-cells (helper T-cells, cytotoxic T-cells, gamma delta T-cells), Bcells,
and natural killer (NK) cells. Primary rat LMC (MLMC) can be used to study the innate and
adaptive immune system.
RLMC from ScienCell Research Laboratories are isolated from healthy adult rat lymph nodes.
RLMC are depleted of erythrocytes, cryopreserved immediately after isolation, and delivered
frozen. RLMC are a mixed population of cells that include T-cells, B-cells, and NK cells. Each
vial contains at least 10 million cells in 1 ml volume. RLMC are quality control tested for viability.
RLMC are negative for mycoplasma, bacteria, yeast, and fungi. RLMC can be maintained for a
limited period of time in culture using the conditions provided by ScienCell Research Laboratories
and are not intended for long term culture.
Recommended Medium
It is recommended to use HematoGro Medium (HeGM, Cat. #5501) for short-term maintenance
of RLMC in vitro.
Product Use
大鼠淋巴单核细胞RLMC are for research use only. They are not approved for human or animal use, or for application
in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and
keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
References
大鼠淋巴单核细胞[1] Willard-Mack C. (2006) “Normal structure, function, and histology of lymph nodes.” Toxicol Pathol 34(5): 409-
424.
Instructions for culturing cells
Caution: Cryopreserved cells are very delicate. Thaw the vial in a 37oC water bath and
return the cells to culture as quickly as possible with minimal handling!
Note: Experiments should be well organized before thawing MC. It is recommended that R MC
are purified or used for experiments as quickly as possible after thawing the cells. Cells are not
intended for long term culture.
Initiating the culture:
1. Prepare complete medium (HeGM, Cat. #5501). Thaw HeGS and P/S solution at 37oC.
Gently tilt the tubes several times to ensure the contents are completely mixed before
adding to the medium. Spray the medium bottle and tubes with 70% ethanol, and wipe to
remove excess liquid. In a sterile field, remove the caps without touching the interior
threads with fingers. Add HeGS and P/S solution to the medium and mix well.
2. Add 15 ml of complete medium to a T-75 flask. Leave the vessel in the sterile field and
proceed to thaw the cryopreserved cells.
3. Place the frozen vial in a 37oC water bath. Hold and rotate the vial gently until the contents
completely thaw. Remove the vial from the water bath promptly, wipe it down with 70%
ethanol and transfer it to the sterile field.
4. Remove the cap carefully without touching the interior threads. Gently resuspend and
dispense the contents of the vial into the equilibrated, culture vessel.
Note: Dilution and centrifugation of cells after thawing are not recommended since these
actions are more harmful to the cells than the effect of residual DMSO in the culture.
5. Replace the cap or lid, and gently rock the vessel to distribute the cells evenly. Loosen cap
if necessary to allow gas exchange.
6. Return the culture vessel to the incubator.
7. Cells should be used promptly for experiments or purified to specifically isolate T-cells
(helper T-cells, cytotoxic T-cells, gamma delta T-cells), B-cells, and NK cells.
Caution: Handling animal derived products is potentially biohazardous. Always wear gloves and
safety glasses when working with these materials. Never mouth pipette. We recommend following
the universal procedures for handling products of human origin as the minimum precaution
against contamination [1].
大鼠淋巴单核细胞[1] Grizzle WE, Polt S. (1988) “Guidelines to avoid personal contamination by infective agents in research laboratories
that use human tissues.” J Tissue Culture Methods. 11: 191-9.