大鼠胚胎成纤维细胞
产品名称： 大鼠胚胎成纤维细胞
英文名称： Rat Embryonic Fibroblast
产品编号： XY7540
产品价格： 0
产品产地： 中国/美国
品牌商标： XYbscience
更新时间： 2023-08-17T09:55:27
使用范围： null
- 联系人 : 徐经理
- 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 152****8802
- 传真 : 021-37680378
- 邮箱 : shxysw02@163.com
大鼠胚胎成纤维细胞Rat Embryonic Fibroblasts (REF) are used to support the growth of rat and human pluripotent stem cells [1]. REF not only provide a substrate for pluripotent stem cells to grow on, but also secrete critical growth factors to maintain stem cell pluripotency. REF are isolated from rat embryos and used at early passages [2]. To serve as feeder cells, REF must be treated with mitomycin C or by irradiation to prevent cell proliferation. The treated cells can also be used to generate conditioned medium for feeder-free culture of pluripotent stem cells.
REF from ScienCell Research Laboratories are isolated from embryonic day 14 rat embryos. These cells are cryopreserved at P0 and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. REF are characterized by immunofluorescence with antibody specific to fibronectin. REF are negative for mycoplasma, bacteria, yeast, and fungi. REF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.
大鼠胚胎成纤维细胞Recommended Medium
It is recommended to use DMEM (Cat. #09221) supplemented with 10% fetal bovine serum (FBS, Cat. #0010, 0025, 0500) for culturing REF in vitro.
| 大鼠胚胎成纤维细胞Catalog No. | R7540 |
|---|---|
| Country of Manufacture | United States |
| Product Code | REF |
| Size/Quantity | 1 x 10^6 cells/vial |
| Product Use | REF are used as feeder layer in rat and human ES or iPS cell culture. They are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| 大鼠胚胎成纤维细胞Shipping Info | Dry ice. |
| References | [1] Bradley, A. (1987). Production and analysis of chimaeras. In Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, (ed. E. J. Robertson), pp. 113-151. Oxford: IRL Press. |