MDA-MB-330细胞

ATCC Number： HTB-127™
数量： 大量
相关疾病： 其他疾病
运输方式： 冻存运输
器官来源： 乳房
是否是肿瘤细胞： 0
物种来源： 人
年限： 43 years
细胞形态： 上皮样
生长状态： 贴壁生长
Designations: MDA-MB-330
Depositors: R Cailleau
Biosafety Level: 1
Shipped: frozen
Medium & Serum:MDA-MB-330细胞See Propagation

Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial

Source: Disease: Infiltrating lobular carcinoma; mammary gland; breast; derived from metastatic site: pleural effusion.
Organ: mammary gland; breast
Derived from Metastatic Site: pleural effusion.
Cellular Products: tubulin; actin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. MDA-MB-330细胞Please click here for information regarding the specific requirements for shipment to your location.

Applications: The population exhibited a mixed phenotype when examined by immunofluorescence staining for tubulin, with some cells (type I) having extensive arrays and others (type II) a more diffuse reaction pattern.
Receptors: epidermal growth factor (EGF)
Tumorigenic: No
Antigen Expression: Blood Type A; Rh+
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 9,12
D5S818: 13
D7S820: 10,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14,16
Cytogenetic Analysis:MDA-MB-330细胞modal number = 64 with one marker chromosome

Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1
PGM3, 1
Age: 43 years
Gender: female
Ethnicity: Caucasian
Comments: The population exhibited a mixed phenotype when examined by immunofluorescence staining for tubulin, with some cells (type I) having extensive arrays and others (type II) a more diffuse reaction pattern.
Most cells of the line stained positively for actin.
This line grows very slowly (doubling time is 10 to 20 days) and never becomes confluent.
Propagation: ATCC complete growth medium: These cells are grown in Leibovitzs L-15 medium with 2 mM L-glutamine supplemented with:
30 ng/ ml human recombinant EGF
0.016 mg/ml human recombinant insulin
2mM glutathione
20% fetal bovine serum
Do not filter EGF thru 0.22 micron filter
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Once per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and let the culture sit at 37C until the cells detach. MDA-MB-330细胞Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 240 to 480 hrs
References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337
22182: Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.
22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779
22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202