SV40LT-SMC Clone HEP-SA细胞

生长状态： 贴壁生长
器官来源： 大动脉
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 大鼠
年限： 3 months
运输方式： 冻存运输
数量： 大量
品系： Sprague-Dawley
ATCC Number： CRL-2018™
组织来源： smooth muscle
细胞形态： 成纤维样
Designations: SV40LT-SMC Clone HEP-SA
SV40LT-SMC Clone HEP-SA细胞Depositors: CF Reilly, R McFall
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: fibroblast

Source: Organ: aorta
Strain: Sprague-Dawley
Tissue: smooth muscle
Cell Type: SV40 transfected
Cellular Products: alpha actin; large T antigen
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Age: 3 months
Comments: This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats.
SV40LT-SMC Clone HEP-SA细胞The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen.
Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance).
Clone HEP-SA cells maintain smooth muscle cell characteristics including smooth cell alpha actin expression and growth inhibition by heparin.
Large T antigen is detectable in the nuclei.
Propagation: ATCC complete growth medium: Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90% supplemented with 0.2 mg/ml G418, 90%; bovine calf serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove. Allow the cells to sit at 37C (or at room temperature) until the cells detach (usually 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liqid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2030
References: 23008: Reilly CF. SV40LT-SMC Clone HEP-SA细胞Rat vascular smooth muscle cells immortalized with SV40 large T antigen possess defined smooth muscle cell characteristics including growth inhibition by heparin. J. Cell. Physiol. 142: 342-351, 1990. PubMed: 2154504