Lym-1细胞

ATCC Number： HB-8612™
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
生长状态： 悬浮生长
细胞形态： 淋巴样
免疫类型： IgG2a
运输方式： 冻存运输
Designations: Lym-1
Depositors: Northwestern University
Isotype: IgG2a
Lym-1细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against normal human B cells and derived malignancies
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Comments: Animals were immunized with preparations of nuclei from Raji cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody stains the surfaces of normal human B cells and derived tumors, but does not react with the surfaces of solid tumors (some cytoplasmic staining is seen with some tumors.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References:Lym-1细胞 3901: Epstein AL. Murine hybridoma Lym-1 and diagnostic antibody produced thereby. US Patent 4,724,213 dated Feb 9 1988