MX2细胞-细胞株/菌种-试剂-生物在线
MX2细胞

MX2细胞

商家询价

产品名称: MX2细胞

英文名称: MX2细胞

产品编号: HZ-CRL-2274™

产品价格: 0

产品产地: 美国

品牌商标: 沪震生物

更新时间: 2023-08-17T10:24:20

使用范围: null

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MX2细胞

细胞形态: 成纤维样
年限: 56 years
器官来源: 子宫
运输方式: 冻存运输
数量: 大量
生长状态: 贴壁生长
是否是肿瘤细胞: 1
物种来源: 人
ATCC Number: CRL-2274™
相关疾病: 其他疾病
Designations: MES-SA/MX2
Depositors: WG Harker
MX2细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast

Source: Organ: uterus
Disease: uterine sarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 13
D16S539: 11,12
D5S818: 13
D7S820: 7,11
THO1: 6
MX2细胞TPOX: 8,12
vWA: 18
Age: 56 years
Gender: female
Ethnicity: Caucasian
Comments: MES-SA/MX2 is a mitoxantrone resistant derivative of the human uterine sarcoma cell line MES-SA (see ATCC CRL-1976).
The line was selected and subcloned in 1988 for resistance to mitoxantrone, an anthracenedione antitumor agent.
These cells were cloned by limiting dilution in soft agar, propagated and tested for sensitively to mitoxantrone.
The clone, designated MES-SA/MX2, was approximately 975 fold less sensitive to mitoxantrone than the parental cells.
The cells display features of both classic multidrug resistance (MDR), P-glycoprotein overexpression and atypical MDR, altered topoisomerase II catalytic activity.
Propagation: ATCC complete growth medium: A 1:1 mixture of Waymouths MB 752/1 medium and McCoys 5a medium, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: MX2细胞Every 2 to 3 days 

These cells grow as a mixture of floating and adherent cells.
Remove the medium with the floating cells, and recover the cells by centrifugation.
Rinse the adherent cells with fresh 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution, and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: parental cell line:ATCC CRL-1976
References: 23048: Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344
23058: Harker WG, Sikic BI. Multidrug (pleiotropic) resistance in doxorubicin-selected variants of the human sarcoma cell line MES-SA. Cancer Res. 45: 4091-4096, 1985. MX2细胞PubMed: 4028002