信号素4B(SEMA4B)重组蛋白

YB336Mu021
Semaphorin Semaphorin Semaphorin Semaphorin 4B (SEMA4B) (SEMA4B) (SEMA4B) (SEMA4B)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
信号素4B(SEMA4B)重组蛋白NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
5th Edition (Revised in January, 2013)
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Semaphorin 4B
Synonyms: Synonyms: Synonyms: Synonyms: SEMA4B, KIAA1745, SEMAC
Species: Species: Species: Species: Human
Size: 100µg
Source: Source: Source: Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Membrane; Single-pass type I
信号素4B(SEMA4B)重组蛋白membrane protein.
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Leu192~Thr367 (Accession # H0YMZ3),
with N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 23kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 5% sucrose, 0.01% sarcosyl. Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by
the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 20.4kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.8
Human SEMA4B
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
信号素4B(SEMA4B)重组蛋白Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- LHKAVSVGP RVHIIEELQI FSSGQPVQNL LLDTHRGLLY
AASHSGVVQV PMANCSLYRS CGDCLLARDP YCAWSGSSCK HVSLYQPQLA
TRPWIQDIEG ASAKDLCSAS SVVSPSFVPT GEKPCEQVQF QPNTVNTLAC PLLSNLATRL
WLRNGAPVNA SASCHVLPTG DLLLVGT