人细胞周期蛋白和周期蛋白依赖性激酶定量PCR芯片试剂盒-PCR/RT-PCR/qPCR-试剂-生物在线
人细胞周期蛋白和周期蛋白依赖性激酶定量PCR芯片试剂盒

人细胞周期蛋白和周期蛋白依赖性激酶定量PCR芯片试剂盒

商家询价

产品名称: 人细胞周期蛋白和周期蛋白依赖性激酶定量PCR芯片试剂盒

英文名称: GQH-CCN

产品编号: GK063

产品价格: 0

产品产地: 美国

品牌商标: sciencell

更新时间: 2024-03-18T09:04:49

使用范围: null

上海中乔新舟生物科技有限公司
  • 联系人 : 胡小姐
  • 地址 : 上海市宝山区长江南路180号A区401-406室
  • 邮编 : 200433
  • 所在区域 : 上海
  • 电话 : 181****0148
  • 传真 : 021-56760351
  • 邮箱 : wwwfudan@163.com;hufangqiong@zqxzbio.com

人细胞周期蛋白和周期蛋白依赖性激酶定量PCR芯片试剂盒描述:

          ScienCells GeneQuery™ Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit (GQH-CCN) is designed to facilitate gene expression profiling of 40 selected cyclins and cyclin-dependent kinases (CDKs). CDKs are a family of small enzymes required for cell cycle progression and cell division, by means of phosphorylation of their substrates. CDKs must bind to cyclins to become fully activated. Cyclin/CDK complexes regulate cell cycle checkpoints and are the driving force of cell cycle progression. Brief examples of how included genes may be grouped are shown below:

  • Cyclins: CCNA, CCNB, CCNC, CCND, CCNE, CCNF, CCNG, CCNH, CCNI, CCNK, CCNL, CCNO, CCNT, CCNY
  • CDKs: CDK1, CDK2, CDK3, CDK4, CDK5, CDK6, CDK7, CDK8, CDK10, CDK20

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Cyclins and Cyclin-dependent Kinases qPCR Array Plate Layout*

(8 controls in Bold and Italic)

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

CCNA1

CCND3

CCNI2

CDK2

CDK11A

β-actin

CCNA1

CCND3

CCNI2

CDK2

CDK11A

β-actin

B

CCNA2

CCNE1

CCNK

CDK3

CDK12

GAPDH

CCNA2

CCNE1

CCNK

CDK3

CDK12

GAPDH

C

CCNB1

CCNE2

CCNL1

CDK4

CDK13

LDHA

CCNB1

CCNE2

CCNL1

CDK4

CDK13

LDHA

D

CCNB2

CCNF

CCNL2

CDK5

CDK15

NONO

CCNB2

CCNF

CCNL2

CDK5

CDK15

NONO

E

CCNB3

CCNG1

CCNO

CDK6

CDK16

PPIH

CCNB3

CCNG1

CCNO

CDK6

CDK16

PPIH

F

CCNC

CCNG2

CCNT1

CDK7

CDK17

GDC

CCNC

CCNG2

CCNT1

CDK7

CDK17

GDC

G

CCND1

CCNH

CCNY

CDK8

CDK19

PPC

CCND1

CCNH

CCNY

CDK8

CDK19

PPC

H

CCND2

CCNI

CDK1

CDK10

CDK20

NTC

CCND2

CCNI

CDK1

CDK10

CDK20

NTC

 

* gene selection may be updated based on new research and development

Catalog No. GK063
Country of Manufacture United States
Product Code GQH-CCN
Size/Quantity One 96-well plate, one 20ul reaction per well
Product Use For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Storage Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer.
Shipping Info Ambient temperature.

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