人心血管疾病定量PCR芯片试剂盒描述：

ScienCells GeneQuery™ Human Cardiovascular Disease qPCR Array Kit (GQH-CVD) is designed to facilitate gene expression profiling of 88 key genes involved in the pathogenesis of heart and coronary artery diseases.  Genes are included but not limited to such as atherosclerosis, myocardial infarction, and ischemic stroke.  Brief examples of how included genes may be grouped according to pathology are shown below:

• Risk factors: APOB, ACE, SELE, SERPINE1, PPARA
• Myocardial infarction: OLR1, LRP8, LGALS2, ITGB3, LTA, F7
• Arteriosclerosis: APOA1, PON1, ADIPOQ, MGP, ICAM1
• Atherosclerosis: CCL2, ABCG8, PPARG, LDLR, APOC2
• Ischemic stroke: NOS3, PTGIS, PLAT, MTHFR

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

GeneQuery™ Human Cardiovascular Disease qPCR Array Plate Layout* (8 controls in Bold and Italic)

 * gene selection may be updated based on new research and development

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