BT-549-CSC-C8211L-item-40994.htm
- Description
- The BT-549 line was isolated in 1978 by W.G. Coutinho and E.Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes. The established population was polymorphic with epithelial like components and multinucleated giant cells. A mucin-like material was secreted into the medium.
- Recommended Medium
- DMEM supplemented with 4.5g/L glucose, 2mM L-glutamine, and 10% FBS
- Organism
- Homo sapiens, human
- STR DNA Profile
- Amelogenin: X, X
CSF1PO: 10, 12
D13S317: 11
D16S539: 8
D5S818: 11
D7S820: 9, 10
THO1: 9,3
TPOX: 8
vWA: 15
D3S1358: 18
D21S11: 32.2
D18S51: 15
Penta E: 14
Penta D: 13
D8S1179: 14, 16
FGA: 19
- Culture Properties
- Adherent
- Karyotype
- modal number = 78; range = 73 to 80.
The cell line is aneuploid human female, with chromosome counts in the hypertriploid range. The X chromosomes are all abnormal. Normal chromosomes N10 and N13 are clearly under-represented, with chromosomes N2, N12, and N17 also tending to be. Chromosome N8 is over-represented with respect to the copy number of other normal chromosomes, and chromosomes N5 and N18 also usually have more copies than most chromosomes. Four marker chromosomes are found: iso(13q), 10q+, del(X)(q22:), del(11)(p11:).
- Quality Control
- Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
- Storage and Shipping
- Liquid nitrogen vapor phase
- Application
- This cell line can be used as a suitable transfection host.
- Disease
- Invasive ductal carcinoma
