分子量:27.6 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Siglec-3, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measuredbytheabilityoftheimmobilizedproteintosupporttheadhesionofhumanredbloodcells. TheED50 forthiseffectistypically0.2-1.2g/mL.

产品特性:Human Siglec-3, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 27.6 kDa. The predicted N-terminus is Asp 18 . The reducing (R) protein migrates as 40-45 kDa in SDS-PAGE  due to glycosylation.

产品背景:Myeloidcellsurfaceantigen CD33 is also known as SIGLEC3, Siglecs(sialicacidbindingIglikelectins) andGP67, is a single-pass type I membrane protein which belongs to theimmunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.