荧光素酶报告基因检测试剂盒上海新睿生物科技有限公司

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产品名称: 荧光素酶报告基因检测试剂盒
英文名称: SuperLight™ Luciferase Reporter Gene Assay Kit
产品货号: SLLU-200
产品规格: 200次检测
产品产地: 美国
产品商标: BioAssay Systems
价    格: 2146元
保存与运输: -20℃
产品资料: 资料下载
更新时间: 2018/1/22 10:09:00
浏览人数:12
诚信指数:65点
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使用范围:仅限科研使用,不能应用于临床
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生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

供应商联系卡

上海新睿生物科技有限公司
地址:
上海市徐汇区蒲汇塘路11号博大商务楼1307室
邮编:
200030
电话:
021-64288052 13818234547
传真:
联系人:
刘君
所在区域:
上海·上海
邮件:
公司展台:
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产品详细描述

 产品名称 :

荧光素酶报告基因测试盒 | SuperLight™ Luciferase Reporter Gene Assay Kit

分类名称 :高效药物筛选产品介绍 :

测定方法   快速(2分钟内)生物发光法测定荧光素酶报告基因在细胞中的表达。

Description    The SuperLightTM Luciferase Reporter Gene Assay is based on the quantitation of luciferase expression in mammalian, yeast or E. coil cells, using luciferin and ATP as substrates. The reaction results in light production which can be conveniently measured on a luminometer Reaction scheme:This bioluminescent reporter gene assay is extremely sensitive and is especially suitable for quantifying luciferase expression in recombinant cells. This ultra-sensitive, homogeneous cell-based assay only requires adding a single reagent to the cells and measuring the light intensity after a short incubation step (2 minutes). Assays can be performed in tubes, cuvettes or multi-well plates. All kit components are compatible with culture media and with all liquid handling systems. With an extended luminescence emission kinetics (half-life 40 min), the SuperLightTM luciferase assays are especially suitable for high-throughput screening in 96-well, 384-well and 1536-well plates. In addition, the reagent provided in the kits has been formulated for maximum sensitivity, reproducibility and long shelf-life. Applications for this kiinclude gene t regulation studies and high-throughput screening of gene modulators.

Key features    High sensitivity and wide detection range: detection of as little of 2 fg luciferase and as few as 4 cells. Plus, the emitted light is linear over seven orders of magnitude.Compatible with routine laboratory and HTS formats: assays can be performed in tubes or microplates, on LJL Analyst, Berthold Luminometer, Top-Count, MicroBeta counters, chemiluminescent image plate readers (CLIPR/LeadSeeker). Assay reagents compatible with all liquid handling systems.Fast and convenient: homogeneous “mix-and-measure” assay allows detection of luciferase levels within 10 minutes. The optimally combined reagent system allows a single addition step, and simultaneous cell lysis and detection.Robust and amenable to HTS: Z’ factors of 0.6 to 0.8 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Applications    Gene Regulation: gene expression level, characterization of promoter activity, modulation of gene expression by receptors, transcription factors and small molecules.Drug Discovery: high-throughput screen for gene modulators.

 

Storage conditions. Store the Reagent in the provided amber tube at -20°C and the Assay Buffer at 2-8°C.  Shelf life: 12 month.

 

 

订货信息 :

编号:

SLLU-200      荧光素酶报告基因检测试剂盒       200次检测         ¥2146

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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