重组人PF-4/CXCL4-蛋白检测-试剂-生物在线
重组人PF-4/CXCL4

重组人PF-4/CXCL4

商家询价

产品名称: 重组人PF-4/CXCL4

英文名称: Recombinant Human Platelet Factor-4/CXCL4

产品编号: HZ600260

产品价格: 0

产品产地: 中国/上海

品牌商标: HZbscience

更新时间: 2023-08-17T10:24:20

使用范围: null

上海沪震实业有限公司
  • 联系人 : 鲍丽雯
  • 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 139****0749
  • 传真 : 021-60345367
  • 邮箱 : www.shzbio.net

 重组人PF-4/CXCL4

附带条件

特技

血小板因子-4(PF- 4)是属于CXC趋化因子家族的小细胞因子,也被称为趋化因子(C-X-MyTf)配体4(CXCL4)。人CXCL4基因位于人类第4号染色体上。这种趋化因子在血小板聚集过程中从活化血小板的α颗粒中释放出来,中和肝素的抗凝作用。它与趋化因子受体CXCR3的剪接变异体相互作用,除了对中性粒细胞、成纤维细胞和单核细胞进行趋化作用外。重组人CXCL4含有70个与肝素亲和力高的氨基酸。具体而言,人类和小鼠CXCL4共享约60%的身份。

附带条件

该材料由Sangon生物技术公司提供,用于研究、实验室或进一步评估目的。不适合人类使用。

附带条件

物理外观无菌过滤白冻干(冻干)粉末。

纯度为97%以上,采用SDS-PAGE和HPLC分析。

来源大肠杆菌。

与标准相比,生物活性完全具有生物活性。用人成纤维细胞的趋化性生物测定法测定的生物活性浓度为1.0~10ng/ml。

用0.2μm过滤浓缩液在20 mm Pb、1.5 mM NaCl、pH 7.4下进行冻干。

LaAl法测定内毒素低于1 EU /μg RHUPF 4/CXCl 4。

我们建议在打开之前将这个小瓶简单地离心,以将内容物带到底部。在无菌蒸馏水或含0.1% BSA的水性缓冲液中重组,浓度为0.1-1.0mg/mL。储备溶液应分配到工作等分试样中,并储存在小于20?c.应在适当的缓冲溶液中进一步稀释。

 

AAA序列EAEDEGDLQC LCVKTTQVVR

产品描述

概述

 

Platelet Factor-4 (PF-4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The gene for human CXCL4 is located on human chromosome 4. This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin. It interacts with a splice variant of the chemokine receptor CXCR3 besides be chemotactic for neutrophils, fibroblasts and monocytes. Recombinant human CXCL4 contains 70 amino acids binds with high affinity to heparin. Specifically, Human and mouse CXCL4 share about a 60 % identity.

使用说明

 

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

技术规格

 

 

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Purity > 97 % by SDS-PAGE and HPLC analyses.
Source Escherichia coli.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human fibroblasts is in a concentration of 1.0-10 ng/ml.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4.
Endotoxin Less than 1 EU/μg of rHuPF-4/CXCL4 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20?C. Further dilutions should be made in appropriate buffered solutions.
AA Sequence EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES