重组小鼠MIF

附带条件

特技

巨噬细胞移动抑制因子（MIF或MMIF），也称为糖基化抑制因子（GIF），L-多巴异构酶，或丙酮酸转互变酶，是由MIF基因编码的蛋白。它通过细菌抗原刺激从白细胞中释放，引起急性免疫反应，或通过糖皮质来对抗糖皮质对免疫系统的抑制作用。MIF是一种同源三聚体，其中每个亚基含有115个氨基酸。如上所述，MIF参与了对细菌病原体的先天免疫应答，并且对抗糖皮质的抗炎活性。此外，它还参与调节巨噬细胞在宿主防御中的功能，并具有体外苯丙酮酸转互变酶和多巴酰转移酶活性。小鼠MIF对人细胞有活性，而人MIF对小鼠细胞有活性。小鼠MIF分别为99%、84%、90%和90% A.A，分别与大鼠、猪、牛和人MIF相同。

附带条件

该材料由Sangon生物技术公司提供，用于研究、实验室或进一步评估目的。不适合人类使用。

附带条件

物理外观无菌过滤白冻干（冻干）粉末。

纯度为96%以上，采用SDS-PAGE和HPLC分析。

来源大肠杆菌。

生物活性生物测定数据是不可用的。

用0.2μm过滤浓缩液在PBS、pH 7.4、1 mm DTT中进行冻干。

LaL法测定内毒素低于EU 1μg。

我们建议在打开之前将这个小瓶简单地离心，以将内容物带到底部。在含0.1% BSA的无菌蒸馏水或含水缓冲液中重组，浓度为0.1-1.0mg/mL。原料溶液应分摊到工作等分试样中，并储存在小于20℃的温度下，应在适当的缓冲溶液中进一步稀释。

AAA序列MPMFVNTNV PRASVPEGFLL TGKPAQYAV HVVPDQLMTF SGTNDPCALC SLHSIGKIGG AQNRNYKSLL CGLLSDRLHI SPRVVYYYY DMANANVGWN GSTFA

概述

Macrophage migration inhibitory factor (MIF or MMIF), also named as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells by bacterial antigen stimulation to trigger an acute immune response, or by glucocorticoids to counter-act the inhibitory effects of glucocorticoids on immune system. MIF is a homotrimer of which each subunit contains 115 amino acids. As mentioned above, MIF is involved in the innate immune response to bacterial pathogens and counter-acts the anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a role as mediator in regulating the function of macrophages in host defense and has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro. Mouse MIF is active on human cells, while human MIF is active on mouse cells. Mouse MIF is 99 %, 84 %, 90 %, and 90 % a.a. identical to rat, porcine, bovine and human MIF, respectively.

使用说明

This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

技术规格