10×PBST

英文名称	PBST,10×
储存条件	2-8°C，有效期至少12个月
外观（性状）	液体
单位	瓶
产品说明：
PBST（phosphate buffered solution）即含吐温-20的磷酸盐缓冲液，能够提供相对稳定的离子环境和pH缓冲能力，是生物学中经常使用的缓冲盐溶液，主要用于免疫组化和原位杂交，酶联免疫等实验中，清洗免疫印迹膜等。产品为无色液体，pH为7.2-7.4，主要成分为磷酸二氢钾、磷酸氢二钠、氯化钠以、氯化钾以及吐温-20。
本产品为10×原液，不可直接使用，需要稀释到1×工作液使用。 4℃保存可延长产品保质期，长期不用建议低温保存。
组分
 浓度
Na2HPO4
 8 mM
NaCl 
0.136M
KH2PO4
2 mM
KCL
2.6 mM
Tween-20
0.5%(V/V)

注意事项：
低温储存较易产生沉淀。如果发现有沉淀产生，请摇晃或置于37℃水浴至完全溶解后再使用。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
参考文献：  《Glutamine Enhances the Hypoglycemic Effect of Insulin in L6 Cells via Phosphatidylinositol-3-Kinase (PI3K)/Protein Kinase B (AKT)/Glucose Transporter 4 (GLUT4) Signaling Pathway》 作者:Caijuan Wang, Yujiao Deng, Yenan Yue, Wenting Chen, Yu Zhang, Guifang Shi, and Zhongming Wu 期刊:Medical Science Monitor 影响因子:1.98 PMID:29491345 《microRNA-1236-3p regulates DDP resistance in lung cancer cells》 作者:Zhigang Wang，Limei Liu，Xiaofeng Guo，Chunmei Guo，Wenxia Wang 期刊:Open Medicine 影响因子:1.221 PMID:30805558 《Escin suppresses HMGB1‐induced overexpression of aquaporin‐1 and increased permeability in endothelial cells》 作者:Changjun Chen， Songgang Wang， Jiying Chen， Xiaolin Liu， Mengchen Zhang， Xi Wang， Weihua Xu，Yayun Zhang， Hao Li， Xin Pan， Meng Si 期刊:FEBS Open Bio homepage 影响因子:1.959 PMID:30972964 《Long non‐coding RNA MALAT1 regulates angiogenesis following oxygen‐glucose deprivation/reoxygenation》 作者:Chengya Wang, Youyang Qu, Rui Suo, Yulan Zhu 期刊:Journal of Cellular and Molecular Medicine 影响因子:4.658 PMID:30784209 《Calycosin stimulates the osteogenic differentiation of rat calvarial osteoblasts by activating the IGF1R/PI3K/Akt signaling pathway》 作者:Yaoyao Fang, Zhiyuan Xue, Lianggong Zhao, Xiuyan Yang, Yafei Yang, Xianglin Zhou, Shilan Feng, Keming Chen 期刊:Cell Biology International 影响因子:1.933 PMID:30632644 《Bone marrow mesenchymal stem cells stimulated with low‐intensity pulsed ultrasound: Better choice of transplantation treatment for spinal cord injury》 作者:Guang‐Zhi Ning， Wen‐Ye Song， Hong Xu， Ru‐Sen Zhu， Qiu‐Li Wu， Yu Wu， Shi‐Bo Zhu， Ji‐Qing Li， Man Wang， Zhi‐Gang Qu， Shi‐Qing Feng 期刊:CNS Neuroscience & Therapeutics 影响因子:4.458 PMID:30294904 《Toxoplasma gondii ROP17 inhibits the innate immune response of HEK293T cells to promote its survival》 作者:Jie-Xi Li,Jun-Jun He,Hany M. Elsheikha,Dan Chen,Bin-Tao Zhai,Xing-Quan Zhu,Hai-Kuo Yan 期刊:Parasitology Research 影响因子:2.067 PMID:30675671 《H2S2-triggered off-on fluorescent indicator with endoplasmic reticulum targeting for imaging in cells and zebrafishes》 作者:Hui Zhou，Jinbao Tang，Lin Sun，Jie Zhang，Bochao Chen，Jianfei Kan，Weifen Zhang，Jian Zhang，Jin，Zhou 期刊:Sensors and Actuators B: Chemical 影响因子:6.393 PMID: 《Efficient Incorporation of Unnatural Amino Acids into Proteins with a Robust Cell-Free System》 作者:Wei Gao , Ning Bu , Yuan Lu 期刊:Methods and protocols 影响因子: PMID:31164598 《MiR-449a regulates the cell migration and invasion of human non-small cell lung carcinoma by targeting ADAM10》 作者:Haining Meng, Qiao Huang, Xijin Zhang,Jiawei Huang,Ruowu Shen, and Bei Zhang 期刊:OncoTargets and Therapy 影响因子:3.046 PMID:31190882 《A deuterohemin peptide protects a transgenic Caenorhabditis elegans model of Alzheimer’s disease by inhibiting Aβ1–42 aggregation》 作者:Jia Xu,Ye Yuan,Ruining Zhang,Yanhui Song,Tianzhuo Sui,Jiaqi Wang,Chonghan Wang,Yujia Chen,Shuwen Guan,Liping Wang 期刊:Bioorganic Chemistry 影响因子:3.926 PMID:30428413 《Neuregulin-1 Accelerates Functional Motor Recovery by Improving Motoneuron Survival After Brachial Plexus Root Avulsion in Mice》 作者:ShuangxiChen,Yuhui Hou,Zhikai Zhao,Yunhao Luo,Shiqin Lv,Qianghua Wang,Jing Li,Liumin He,Libing Zhou,Wutian Wu 期刊:Neuroscience 影响因子:3.244 PMID:30731156 《Regulatory mechanisms of miR-96 and miR-184 abnormal expressions on otic vesicle development of zebrafish following exposure to β-diketone antibiotics》 作者:Jieyi Li,Yuhang Ling,Wenhao Huang ,Limei Sun,Yanyan Li,Caihong Wang,Yuhuan Zhang,Xuedong Wang,Randy A.Dahlgrend,Huili Wang 期刊:Chemosphere 影响因子:5.108 PMID:30265930 "《The Proangiogenic Roles of Long NonCoding RNAs Revealed by RNA-Sequencing Following Oxygen-Glucose Deprivation/ReOxygenation》 作者:Chengya Wang ,Youyang Qu ,Di Wang ,Yulan Zhu 期刊:Cellular Physiology and Biochemistry 影响因子:5.5 PMID:30921509" 《Diagnosis of hepatocellular carcinoma using a novel anti?glycocholic acid monoclonal antibody?based method》 作者:Miao Wu, Songhui Zhai, Julia Gao, Dapeng Wei, Jianxin Xue, Yuxi Zhou, Nan Li, Lijuan Hu 期刊:Oncology Letters 影响因子:1.871 PMID:30867740 《l-Proline Alleviates Kidney Injury Caused by AFB1 and AFM1 through Regulating Excessive Apoptosis of Kidney Cells》 作者:Huiying Li , Songli Li, Huaigu Yang ,Yizhen Wang , Jiaqi Wang , and Nan Zheng 期刊:Toxins 影响因子:3.895 PMID:30995739 《Silencing of triazophos-induced Hexokinase-1-like reduces fecundity in Nilaparvata lugens (St?l) (Hemiptera: Delphacidae)》 作者:Lin-Quan Ge,Hao-Tian Gu,Xin Li,Sui Zheng,Ze Zhou,Hong Miao,Jin-Cai Wu 期刊:Pesticide Biochemistry and Physiology 影响因子:2.87 PMID:30744893