PHA上海逍鹏生物科技有限公司

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产品名称: PHA
英文名称: Phytohemagglutinin-M (PHA-M), Liquid
产品货号: 12-009-1
产品规格: 5ml
试剂级别: 细胞培养级
产品产地: 以色列
产品商标: Biological industries(BI)
价    格: 询价
保存与运输: 根据产品特性
现货状态: 充足
产品资料: 点击查看
更新时间: 2019/10/18 10:12:00
浏览人数:10
诚信指数:1021点
了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
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供应商联系卡

上海逍鹏生物科技有限公司
地址:
上海市浦东新区祖冲之路1505弄80号1幢3楼
邮编:
200120
电话:
021-58785545,13585960425
传真:
021-58777053
联系人:
贾经理
所在区域:
上海·浦东
邮件:
公司展台:
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产品详细描述

 Product Description:

Although lectins may be isolated from a large array of plants, microorganisms, invertebrates, lower vertebrates and from mammalian cell membranes, Phytohemagglutinin-M (PHA-M) is a compound extracted from the red kidney bean herbaceous angiosperm, (Phaseolus vulgarus). Lectins, some of which are also known as hemagglutinins, are proteins or glycoprotein substances of a non-immune nature, capable of specific recognition of and reversible binding to carbohydrate moieties of complex glyco-conjugates without specifically altering the covalent structure of any of the recognized glycosyl ligands. Lectins may also show a characteristically high specificity for attachment to individual carbohydrate–binding molecules (e.g. sugar moieties) or may bind to a soluble carbohydrate or to a CHO moiety which is part of a glycoprotein or glycolipid complex. They may also cross-link themselves in cell walls or membranes thereby changing the physiology of the membrane per se causing polyagglutination of many different mammalian red blood cell types, altering cell membrane transport systems, altering cell permeability to the ingress of proteins, generally interfering with cellular metabolism, inducing mitosis or other cell biochemical changes to name a few.

 

Although each batch of PHA-M is tested for performance, the use of Biological Industries PHA-M does not guarantee the  successful outcome of any diagnostic testing.

 

Instructions/Procedure:

1) Take a vial of Phytohemagglutinin-M (PHA-M) from the specified storage conditions at  2-8°C and read the label.

2) Defrost the PHA-M at room temperature or at 2-8°C. After thawing, each ml will contain 5-10mg of protein.

3) Using aseptic/sterile technique under a laminar-flow culture hood , inoculate approximately 0.5ml of heparinized whole blood into a glass or plastic conveyance with 10ml of medium or in toto,106 viable cells per ml.

4) Incubate the culture at 37°C in a 5% CO2 atmosphere for 72 hours.

5) Recommended Use: two-four (2-4) ml of re-hydrated PHA-M per 100ml of culture medium.

 

产品官网链接:https://www.bioind.com/worldwide/phytohemagglutinin-m-pha-m-liquid/

说明书下载:英文说明书

 

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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