性能参数
产品详细描述
Product Description：
Colcemid, N-deacetyl-N-Methylcolchicine, is related to colchicine, but animal studies found it to be much less toxic. Colcemid arrests mitotic cultured cells in metaphase and it should be treated carefully, since it is mutagenic, tumorigenic, and teratogenic. Colcemid Solution from Biological Industries is prepared in phosphate buffered saline (PBS) and it is recommended to use a concentration of 0.1 µg/ml in culture medium. Colcemid is recommended for use in chromosome analysis during lymphocyte karyotyping and amniotic fluid cell chromosome analysis, and in cell synchronization. Store the Colcemid Solution at 2-8ºC, protected from light.
Suggested Procedure：
1. Chromosome Analysis
Amniotic fluid cells, Peripheral blood and bone marrow cells after initial cultivation. Colcemid Solution arrests mitotic cells in metaphase.
Suggested concentration is 0.1-0.2µg/ml.
2. Cell Synchronization
Colcemid Solution aids in determining the mitotic activity index for cell synchronization studies. Colcemid has been shown to inhibit HeLa and CHO cells at concentrations of 0.05 – 0.5µg/ml.
产品官网链接：https://www.bioind.com/worldwide/colcemid-demecolcine-solution/
查看 秋水酰胺 产品的用户还对以下产品感兴趣
PeproTech PeproGrow™hESC（胚胎干细胞）培养基 [供应商：美国PeproTech(派普泰克)公司中国代表处]
PeproTech PeproGrow™hESC Plus（胚胎干细胞）培养基 [供应商：美国PeproTech(派普泰克)公司中国代表处]
L-Alanyl-L-Glutamine (Stable Glutamine) (200 mM) [供应商：上海逍鹏生物科技有限公司]
羊水细胞培养基 [供应商：上海逍鹏生物科技有限公司]
骨髓细胞培养基 [供应商：上海逍鹏生物科技有限公司]
造血细胞培养基 [供应商：上海逍鹏生物科技有限公司]
细胞遗传学-胰酶 [供应商：上海逍鹏生物科技有限公司]
外周血淋巴细胞培养基 [供应商：上海逍鹏生物科技有限公司]
低渗溶液氯化钾 [供应商：上海逍鹏生物科技有限公司]
秋水酰胺 [供应商：上海逍鹏生物科技有限公司]
