Colcemid, N-deacetyl-N-Methylcolchicine, is related to colchicine, but animal studies found it to be much less toxic. Colcemid arrests mitotic cultured cells in metaphase and it should be treated carefully, since it is mutagenic, tumorigenic, and teratogenic. Colcemid Solution from Biological Industries is prepared in phosphate buffered saline (PBS) and it is recommended to use a concentration of 0.1 µg/ml in culture medium. Colcemid is recommended for use in chromosome analysis during lymphocyte karyotyping and amniotic fluid cell chromosome analysis, and in cell synchronization. Store the Colcemid Solution at 2-8ºC, protected from light.
1. Chromosome Analysis
Amniotic fluid cells, Peripheral blood and bone marrow cells after initial cultivation. Colcemid Solution arrests mitotic cells in metaphase.
Suggested concentration is 0.1-0.2µg/ml.
2. Cell Synchronization
Colcemid Solution aids in determining the mitotic activity index for cell synchronization studies. Colcemid has been shown to inhibit HeLa and CHO cells at concentrations of 0.05 – 0.5µg/ml.
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