造血细胞培养基上海逍鹏生物科技有限公司

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产品名称: 造血细胞培养基
英文名称: BIO-HEMATO™ Karyotyping Medium, with conditioned medium
产品货号: 01-200-1
产品规格: 100ml
试剂级别: 细胞培养级
产品产地: 以色列
产品商标: Biological industries(BI)
价    格: 询价
保存与运输: 根据产品特性
现货状态: 充足
产品资料: 点击查看
更新时间: 2019/10/18 10:12:00
浏览人数:12
诚信指数:1021点
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使用范围:仅限科研使用,不能应用于临床
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上海逍鹏生物科技有限公司
地址:
上海市浦东新区祖冲之路1505弄80号1幢3楼
邮编:
200120
电话:
021-58785545,13585960425
传真:
021-58777053
联系人:
贾经理
所在区域:
上海·浦东
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产品详细描述

 Culture of Bone Marrow and Peripheral Blood Cells for Chromosome Analysis 

The hematopoietic cell karyotyping method was developed to provide information about chromosomal abnormalities. In the presence of a conditioned medium, acute and chronic nonlymphocytic leukemic cells in bone marrow and peripheral blood cultures are stimulated to enter into mitosis by DNA replication. After 48-72 hours, a mitotic inhibitor is added to the culture to stop mitosis in the metaphase stage. After treatment by hypotonic solution, fixation and staining, chromosomes can be microscopically observed and evaluated for abnormalities.


Test Procedure 

1. Inoculate approximately 0.5ml of bone marrow suspension or 0.5-1x107 Ficoll-separated peripheral blood cells into a plastic tube or tissue culture plate with 10ml of medium. Invert tubes gently to mix specimen. 

2. Incubate the culture for 72-120 hours.

3. Add 0.1-0.2ml of Colcemid Solution (Cat. No. 12-004- 1) to each culture tube. Incubate the culture for an additional 15-30 minutes. 

4. Transfer the culture to a centrifuge tube and spin at 500g for 5 minutes. 

5. Remove the supernatant and re-suspend the cells in 5-10ml of hypotonic 0.075M KCl (Cat. No. 12-005-1). Incubate at 37ºC for 10-12 minutes. 

6. Spin at 500g for 5 minutes. 

7. Remove the supernatant, agitate the cellular sediment and add drop-by-drop 5-10ml of fresh, ice-cold fixative made up of 1 part acetic acid to 3 parts methanol. Leave in 4ºC for 10 minutes. 

8. Repeat steps 6 and 7. 

9. Spin at 500g for 5 minutes. 

10. Re-suspend the cell pellet in a small volume 0.5-1ml of fresh fixative, drop onto a clean slide and allow to air dry. 

11. At this stage, the preparation can be stained with Orecin or Giemsa. Giemsa banding has become the most widely used technique. The most common method to obtain this staining is to treat slides with Trypsin-EDTA 10X (Cat. No. 03-051-5).

 

产品官网链接:https://www.bioind.com/worldwide/bio-hemato-medium-with-conditioned-medium/#

说明书下载:造血细胞培养基英文说明书

 

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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