超敏ECL发光液
产品名称： 超敏ECL发光液
英文名称： 超敏ECL发光液
产品编号： HZ2301
产品价格： 0
产品产地： 中国/上海
品牌商标： 沪震生物
更新时间： 2023-08-17T10:24:20
使用范围： null
上海沪震实业有限公司
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749
- 传真 : 021-60345367
- 邮箱 : www.shzbio.net
超敏ECL发光液
| Western Blot结果的好坏与否与ECL发光液的选择有着密切关系，好的ECL发光液不仅可曝光得到漂亮的目的条带，还可节省昂贵的抗体。海基超敏ECL发光液用于Western、Northern和Southern blot分子杂交的化学发光检测，原理是采用新型发光底物（Luminol），其与辣根过氧化物酶反应时产生很强的发光信号，以此检测微量蛋白而获得理想的实验结果。比传统化学显色法灵敏度高，可达pg水平，发光持续时间长，信号30min内不会发生明显降低。 |  |
||||||||||
组分 |
|||||||||||
|
|||||||||||
应用 |
|||||||||||
| 在Western Blot印迹分析，核酸杂交以及EMSA实验中，HRP标记的二抗或探针与靶分子结合再与底物反应产生可见光。 | |||||||||||
储存 |
|||||||||||
| 2-8°C，Super ECL A要求避光保存。 | |||||||||||
实例 |
|||||||||||
 |
 |
||||||||||
| Fig1.检测Hela细胞裂解物的GAPDH蛋白，应用超敏ECL发光液显色，一抗为兔抗GAPDH多克隆抗体，稀释比例为1:1000。 | Fig2.检测A549细胞裂解物的AKT蛋白，应用超敏ECL发光液和Competitor P公司的ECL发光液分别显色， 一抗为AKT rabbit mab(CST,#4691)，稀释比例为1:1000。 |
||||||||||
相关产品 |
|||||||||||
应用本产品文献 |
|||||||||||
| 1.Calcium oxalate crystals induces tight junction disruption in distal renal tubular epithelial cells by activating ROS/Akt/p38 MAPK signaling pathway. 2.Nrf2 mediates the protective effects of homocysteine by increasing the levels of GSH content in HepG2 cells. 3.EHD1 confers resistance to cisplatin in non-small cell lung cancer by regulating intracellular cisplatin concentrations. 4.Nuclear factor erythroid 2-related factor 2 activation mediates hyperhomocysteinemia-associated lipolysis suppression in adipocytes. 5.Protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating PINK1/Parkin pathway and BNIP3L. 6.Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content. 7.The Wnt/β-Catenin/LEF1 Pathway Promotes Cell Proliferation at Least in Part Through Direct Upregulation of miR-17-92 Cluster. 8.Membrane-permeabilized sonodynamic therapy enhances drug delivery into macrophages. 9.MicroRNA‐125a inhibits tumorigenesis by targeting Smurf1 in colorectal carcinoma. 10.Double Knockdown of PHD1 and Keap1 Attenuated Hypoxia-Induced Injuries in Hepatocytes. 11.Soluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells. 12.LRH1 as a promising prognostic biomarker and predictor of metastasis in patients with non‐small cell lung cancer. 13.Wnt4 is significantly upregulated during the early phases of cisplatin-induced acute kidney injury. 14.CCAT2 is an oncogenic long non-coding RNA in pancreatic ductal adenocarcinoma. 15.Chronic Moderate Alcohol Intakes Accelerate SR-B1 Mediated Reverse Cholesterol Transport. 16. Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway. 17.Tissue cholesterol content alterations in streptozotocin-induced diabetic rats. 18.Arsenic trioxide promotes senescence and regulates the balance of adipogenic and osteogenic differentiation in human mesenchymal stem cells. 19.STAT1 negatively regulates hepatocellular carcinoma cell proliferation. 20.Preparation and Characterization of a Novel Aspirin Derivative with Anti-Thrombotic and Gastric Mucosal Protection Properties. 21.Effects of hepatocyte growth factor onMMP-2 expression in scleral fibroblasts from a guinea pig myopia model. 22.Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells. |
|||||||||||
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！