磷酸钙转染试剂盒
产品名称: 磷酸钙转染试剂盒
英文名称: Calcium Phosphate Transfection Kit
产品编号: CAPHOS-1KT
产品价格: 0
产品产地: 美国
品牌商标: Merck
更新时间: null
使用范围: null
北京百奥创新科技有限公司
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Application: Calcium Phosphate Transfection Kit has been used: to enable transfection. to transfect Hek293T cells. to transfect H29D cells. Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method: BAEC; Bowels melanoma cells; CHO K1; COS-7; Fibroblasts (human embryonic, neo derm); HEK293; Huh 7; IMR-90; LLC (Lewis Lung Carcinoma); NIH3T3; PC-12; PCI-13; SH-Sy5Y; SK-Hep-1; T47D. Features and Benefits: Suitable for transient and stable transfection. Reproducible for a wide range of cell types. Widely referenced. Inexpensive. Components: The Calcium Phosphate Transfection Kit contains: 5 ml 2.5M CaCl2 (C2052) 25 ml 2x HEPES Buffered Saline (H1012) 25 ml molecular biology grade water (W4502) Principle: The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl2 solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.