We offers full Technology-Transfer packages, which include a comprehensive course at our facility. The company has developed a simple, cost effective and easy to implement proprietary Technology for the production of highly active bulk recombinant E.Coli derived proteins.
Each batch production at our facility is within 2-3 days and can yield 1000-2000 mg/protein.
The Technologies offered give a clear edge over the competition resulting from low production costs, high yield and production efficiency.
We are also seeking for strategic partners and alliances in the biotech and pharmaceutical field to develop and market bio-drugs according to FDA and EU pharma regulations and certification.
We offer the following recombinant technologies:
Interferon gamma 1b
Granulocyte Colony Stimulating Factor
Granulocyte Macrophage Colony Stimulating Factor
*** Like Growth Factor
Epidermal Growth Factor
Fibroblast Growth Factor
For more technology offers please contact us at email@example.com
Brief description of technology transfer package:
2.2 Construction of the plasmid expressing of the protein.
2.3 DNA sequence.
2.4 Expression of the protein in E.coli.
3. Biosynthesis of the protein substance.
3.1 Fermentation process:
3.3 Seed fermentor.
3.4 Production fermentor.
3.5 Fermentor Harvest.
3.6 Cell disruption.
4. Purification of the protein from inclusion bodies
4.1 Dissolution of the protein inclusion bodies.
4.2 Dilution and refolding of the protein.
4.3 Clarification and concentration.
4.4 Enzymatic removal of the N-terminal methionine (if needed).
4.5 Purification of the protein by preparative RP-HPLC (if needed).
4.6 Purification of the protein by ion-exchange Chromatography (if needed).
4.7 Dialysis and Lyophilization.
5. Detailed characterization of the protein
5.1 Polyacrylamide gel electrophoresis.
5.2 UV spectroscopy.
5.3 N-terminal amino acid sequence determination.
5.4 Analytical reversed-phase HPLC.
5.5 Biological activity in-vitro.
6. Characterization of impurities
6.1 Endotoxin content by LAL.
If you have any requirement, please don't hesitate to contact us!
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