Rat hypoxia-inducible factor 1α,HIF-1α ELISA Kit使用原理
Rat hypoxia-inducible factor 1α,HIF-1α ELISA Kit使用原理The kit was a solidphase sandwich enzyme-linked immunosorbent assay (ELISA), and the AVPconcentrations of the standard samples and unknown concentrations ofsamples were detected by the addition of microporous enzyme plates.. Atthe same time, the AVP and the biotin labeled antibodies were incubatedat the same time.. After washing, the addition of Pro labeled HRP. Afterthe warming and washing, the removal of the non binding enzymeconjugate, and then added to the substrate a, B, and the enzymeconjugate at the same time. Produce color. The color depth and theconcentration of AVP in the sample was proportional to the concentrationof the sample..
1 reagent should be stored in the label manual, before using to roomtemperature. The standard after dilute dilute should be discarded, donot save.
2 the experimental plate should be immediately put back in the bag,seal the preservation, lest metamorphism.
3 no other reagents should be packaged or well. Do not mix differentbatches of reagents. Use before the shelf.
4 use a disposable suction head lest cross contamination, draw thetermination liquid and substrate B, a liquid, to avoid using the metalpart of the sample.
5 use clean plastic containers to configure the washing liquid. Allsamples of ingredients and mix well before use in the kit.
6 wash the enzyme plate should be fully dry, do not absorb water paperdirectly into the enzyme labeled pores in the water absorption.
7 substrate a should be volatile, to avoid prolonged open lid.Substrate B is sensitive to light, avoiding prolonged exposure to light.To avoid contact with hand, toxic. After the experiment, read the ODvalue immediately..
8 add reagents should be consistent in order, to ensure that allreaction plate hole incubation time.
9 to carry out the temperature fertility operation according to thetime, the amount and the order of the liquid added in the instruction manual.
1 the correlation coefficient r value of linear regression and theexpected concentration of sample was 0.92 above.
The 2 batch and the batch should be less than 9% and 15% respectively.
1.5 -90 g/ml g/ml
Save condition and its validity:
1 reagent kit preservation: 2-8.
2 validity: 6 months
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