Anti-EGFR (G863D) Mouse Monoclonal Antibody-抗体-抗体-生物在线
武汉纽斯特生物技术有限公司
Anti-EGFR (G863D) Mouse Monoclonal Antibody

Anti-EGFR (G863D) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-EGFR (G863D) Mouse Monoclonal Antibody

英文名称： Anti-EGFR (G863D) Mouse Monoclonal Antibody

产品编号： 26119

产品价格： null

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IF、IHC

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EGFR (G863D) [26119]
[100 µL]		  

Catalog Number:  26119
Gene Symbol:  EGFR/HER1/ERBB1
Description:   Anti-EGFR (G863D) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of G863D, human origin.
Tested Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:1000-1:5000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR (G863D), but not wild type EGFR from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
 
Western blot:  
Western blot analysis of recombinant EGFR (G863D) and wildtype proteins. Purified His-tagged EGFR (G863D) protein (amino acid 702-987) (Cat. #10222) and corresponding wild-type protein (Cat. #10210) were blotted with anti-EGFR (G863D) mouse monoclonal antibody (Cat. #26119). Proteins loading were blotted with anti-His-tag monoclonal antibody (Cat. #28004).
Immunofluorescence:  
Immunofluorescence of cells expressing EGFR proteins with anti-EGFR (G863D) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP-EGFR (G863D) plasmid (right column), then fixed and stained with anti-EGFR (G863D) monoclonal antibody (Cat. #26119).
Publications:
1.  Immunohistochemistry with a novel mutation-specific monoclonal antibody as a screening tool for the EGFR L858R mutational status in primary lung adenocarcinoma
    Tumor Biology. October 2014