CPSF2,剪切及多聚腺苷酸化特异因子蛋白2抗体

产品编号HZ-14036R
英文名称CPSF2
中文名称剪切及多聚腺苷酸化特异因子蛋白2抗体
别 名Cleavage and polyadenylation specific factor 2; Cleavage and polyadenylation specificity factor 100 kDa subunit; Cleavage and polyadenylation specificity factor subunit 2; CPSF 100 kDa subunit; CPSF 2; CPSF100; KIAA1367.
说 明 书0.1ml 0.2ml
研究领域染色质和核信号 细胞分化 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Cow, Horse, Rabbit,
CPSF2,剪切及多聚腺苷酸化特异因子蛋白2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量88kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CPSF2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CPSF2,剪切及多聚腺苷酸化特异因子蛋白2抗体PubMedPubMed
产品介绍background:
CPSF contains four subunits and localizes to the nucleoplasm where it recognizes the AAUAAA signal in pre-mRNA and interacts with other proteins to facilitate RNA cleavage and poly(A) synthesis (1,4,5). The human CPSF2 gene maps to chromosome 14q32.12 and encodes the second largest subunit of cleavage and polyadenylation specificity factor. U1 snRNP-A protein (U1A) interacts with and affects the activity of CPSF by stabilizing the interaction of CPSF with the AAUAAA-containing RNAs to increase the efficiency of polyadenylation .

Function:
CPSF2 belongs to the RNA-metabolizing metallo-beta-lactamase-like family, CPSF2/YSH1 subfamily. It is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3 prime-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. A poly(A) tail is added to eukaryotic mRNA precursors after tightly coupled cleavage and polyadenylation reactions at their 3-prime ends.

Subcellular Location:
Nuclear.

Tissue Specificity:

Gene ID:
53981

Database links:
Entrez Gene: 53981 Human
Entrez Gene: 51786 Mouse
Entrez Gene: 299256 Rat
Omim: 606028 Human
SwissProt: Q9P2I0 Human
SwissProt: O35218 Mouse
SwissProt: D3Z9E6 Rat
Unigene: 657632 Human

CPSF2,剪切及多聚腺苷酸化特异因子蛋白2抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.