beta-Tubulin(10B1)小鼠单克隆抗体 (M20005)-仪器资料-资讯-生物在线

beta-Tubulin(10B1)小鼠单克隆抗体 (M20005)

作者:上海纽普生物科技有限公司 2014-09-22T00:00 (访问量:2794)

 

概述

  • 产品名称beta-Tubulin(10B1)小鼠单克隆抗体
  • 产品描述
    该beta-Tubulin抗体是用人beta-tubulin蛋白的C端蛋白片段免疫小鼠制备而成的单克隆抗体。β-Tubulin即微管蛋白,是细胞的一种骨架蛋白。Tubulin分为&alpha
  • 测试过的应用IF,WB
  • 种属反应性人,猴子,小鼠,大鼠
  • 别名
    -

属性

  • 产品形式液体
  • 保存说明4°C短期保存(1-2周),于-20°C或者更低温度长期保存,自发货日起1年内稳定。分装避免反复冻融。
  • 保存缓冲液1×PBS, pH 7.2, 0.05%叠氮化钠
  • 浓度50 μg at 1 mg/ml
  • 纯度Protein A/G纯化
  • Clonality单克隆
  • 研究领域信号转导,标签/细胞标记物,同型/内参

应用

Western blotting 1:5000
Immunofluorescence 1:1000

验证数据

Western blot analysis of HeLa cell lysate, using Abmart beta-Tubulin (10B1) Mouse mAb. The antibody dilutions are 1:2000 (lane 1), 1:5000(lane 2) and 1:10000 (lane 3). Each lane was loaded with 10 μg of cell lysate.
IF analysis of HeLa cells, NIH/3T3 cells, COS-7 cells and 293T cells, using Abmart beta- Tubulin (10B1) Mouse mAb at a 1:1000 dilution. Red: anti-beta-Tubulin; Blue: DAPI Primary antibody 1: 500

参考文献

1.Weisenberg, R. 1981. Invited review: the role of nucleotide triphosphate in Actin and Tubulin assembly and function. Cell Motil. 1: 485-497.
2. Hoffman, P.N. 1988. Distinct roles of neurofilament and Tubulin gene expression in axonal growth. Ciba Found. Symp. 138: 192-204.
3. Zheng, Y., Jung, M.K. and Oakley, B.R. 1991. γ Tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome. Cell 65: 817-823.
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