钙黄绿素活性染料选择指南-分析方法-资讯-生物在线

钙黄绿素活性染料选择指南

作者:西安百萤生物科技有限公司 2023-05-19T00:00 (访问量:2623)

 钙黄绿素 AM 是一种膜渗透性荧光酯酶底物,通常用于检测细胞活性和质膜完整性。作为一种近中性的底物,钙黄绿素 AM 可以自由、无创地扩散穿过质膜并渗透到活细胞的胞质溶胶中。一旦进入细胞内,代谢活跃细胞中的胞质酯酶会迅速将非荧光钙黄绿素 AM 底物水解成钙黄绿素产物,并由具有完整质膜的细胞保留。由于死细胞缺乏酯酶活性,因此仅标记活细胞。在蓝光激发下,钙黄绿素发出强烈的绿色荧光,与细胞酯酶的活性和样品中存在的活细胞数量成正比。与其他活细胞标记试剂(如 BCECF AM 或羧基荧光素二乙酸酯)相比,钙黄绿素 AM 荧光更亮、光稳定性更好,低细胞毒性有利于细胞追踪、细胞凋亡和细胞毒性的研究。

 

      AAT Bioquest 提供多种可供选择的用于细胞活力分析的钙黄绿素 AM 产品。除了传统的绿色荧光钙黄绿素 AM 之外,我们还提供发出蓝色、橙色、红色和深红色荧光的钙黄绿素 AM 衍生物。

 

钙黄绿素活性染料选择指南

Dye

Calcein Blue

CytoCalcein™ Violet 450

Calcein UltraBlue™

CytoCalcein™ Violet 500

Calcein UltraGreen™

Calcein

Calcein Orange™

Calcein Red™

Calcein Deep Red™

Principle

In live cells, non-fluorescent calcein AM is converted to a fluorescent calcein product after AM ester hydrolysis by intracellular esterases.

Readout

Live cells are highly fluorescent and easily distinguishable from dead cells, which are non-fluorescent.

Laser(nm)

UV (350)

Violet (405)

UV (350)

Violet (405)

Blue (488)

Blue (488)

Green (532)

Yellow (561-568)

Red (633-647)

Ex/Em(nm)

354/441

406/445

359/458

420/505

492/514

501/521

531/545

562/576

643/663

Multiplexable

Yes

Yes

Yes

Yes

Yes

Yes

Yes

Yes

Yes

Dye Compatibility

Compatible with green and red fluorescent proteins, indicators, and other probes

Compatible with green and red fluorescent proteins, indicators, and other probes

Compatible with green and red fluorescent proteins, indicators, and other probes

Compatible with blue and red fluorescent proteins, indicators, and other probes

Compatible with blue and red fluorescent proteins, indicators, and other probes

Compatible with blue and red fluorescent proteins, indicators, and other probes

Compatible with blue and red fluorescent proteins, indicators, and other probes

Compatible with blue and green fluorescent proteins, indicators, and other probes

Compatible with blue and green fluorescent proteins, indicators, and other probes

Sample Type

Live Cells

Platform

Fluorescence Microscope, Fluorescence Microplate Reader, Flow Cytometer

Cat No.

AM Ester

22007

22012

21908

22013

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