Anti-phospho-PKC zeta (Thr560)抗体-抗体-抗体-生物在线
Anti-phospho-PKC zeta (Thr560)抗体

Anti-phospho-PKC zeta (Thr560)抗体


产品名称: Anti-phospho-PKC zeta (Thr560)抗体

英文名称: phospho-PKC zeta (Thr560)

产品编号: YB--12690R

产品价格: null

产品产地: 中国/美国

品牌商标: Ybscience

更新时间: 2023-08-17T10:29:50

使用范围: 科研使用

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 Anti-phospho-PKC zeta (Thr560)抗体

产品编号 YB-12690R
英文名称 phospho-PKC zeta (Thr560)
中文名称 磷酸化蛋白激酶C zeta 抗体
别    名 PKC zeta (phospho T560); p-PKC zeta (phospho T560); 14-3-3-zetaisoform; AI098070; aPKCzeta; C80388; EC; KPCZ_HUMAN; nPKC zeta; nPKC-zeta; OTTHUMP00000001368; OTTHUMP00000044160; PKC 2; PKC ZETA; PKC2; Pkcz; PKCZETA; PKM-zeta, included; PRKCZ; Protein kinase C zeta; Protein kinase C zeta form; Protein kinase C zeta type; r14-3-3; R74924; zetaPKC.  
规格价格 100ul/1580元 购买        大包装/询价
说 明 书 100ul  
产品类型 磷酸化抗体 
研究领域 肿瘤  细胞生物  信号转导  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 67kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human PKC zeta around the phosphorylation site of Thr560:QL(p-T)PD 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.

Forms a ternary complex with SQSTM1 and KCNAB2.

Subcellular Location:
Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.

Tissue Specificity:
Expressed in brain, and to a lesser extent in lung, kidney and testis.

Post-translational modifications:
CDH5 is required for its phosphorylation at Thr-410.

Belongs to the protein kinase superfamily. 
AGC Ser/Thr protein kinase family. 
PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.


Gene ID:

Database links:

Entrez Gene: 5590 Human

Entrez Gene: 18762 Mouse

Entrez Gene: 25522 Rat

Omim: 176982 Human

SwissProt: Q05513 Human

SwissProt: Q02956 Mouse

SwissProt: P09217 Rat

Unigene: 496255 Human

Unigene: 28561 Mouse

Unigene: 1109 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
A549 (Human) CellLysate at 40 ug
293T (Human) CellLysate at 40 ug
Primary: Anti-p-PKC zeta (Thr560)(bs-12690R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 67kD
Observed band size: 67kD