RasProteinQ61Lmutant-蛋白质/抗原/多肽-试剂-生物在线
武汉纽斯特生物技术有限公司
RasProteinQ61Lmutant

RasProteinQ61Lmutant

商家询价

产品名称： RasProteinQ61Lmutant

英文名称： RasProtei Q61Lmutant

产品编号： 10111

产品价格： 0

产品产地： 美国

品牌商标： NewEastBio

更新时间： null

使用范围： null

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Ras Protein Q61L mutant [10111]
[25 µg]		  
Catalog Number:  10111
Product Name:  Ras Protein Q61L mutant
Synonyms:  GTPase Ras
Source:   Human, recombinant full length, His6-tag
Expression Host:   E. coli
Molecular Weight:   21 kDa
Purity:   >95% by SDS-PAGE
Introduction:   Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
Amino Acid Sequence   (1-189, Q61L)
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGLEEYSAM 
RDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLA 
RSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):  White or clear
Concentration:  3 mg/mL
Storage:   -80°C
Preparation Instructions:  
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Ras Q61L was determined by SDS-PAGE and Coomassie Brilliant Blue Staining.
References:  
1. Chen, H.-J. et al., Neuron 20: 895-904, 1998. 
2. Hamdan, F. F. et al., New Eng. J. Med. 360: 599-605, 2009. 
3. Oh, J. S. et al., Neuron 33: 151 only, 2002. 
4. Rumbaugh, G. et al., Proc. Nat. Acad. Sci. 103: 4344-4351, 2006. 
5. Tomoda, T. et al., Genes Dev. 18: 541-558, 2004.
Datasheet:  
Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
    Gene Therapy 21, 888-896 (October 2014)