Extracellular Death Factor 小鼠单克隆抗体
产品名称： Extracellular Death Factor 小鼠单克隆抗体
英文名称： Extracellular Death Factor Mouse monoclonal antibody
产品编号： LPMA10233
产品价格： null
产品产地： 中国
品牌商标： Ablepscience
更新时间： null
使用范围：
- 联系人 :
- 地址 : 武汉东湖新技术开发区汤逊湖北路36号新能源研发基地3号楼3层314室
- 邮编 : 430075
- 所在区域 : 湖北
- 电话 : 027-818*5800 点击查看
- 传真 : 点击查看
- 邮箱 : sale@apescience.cn;3055949650@qq.com
产品索引
Description: mazEF is a toxin-antitoxin stress-induced module which is abundant on the chromosome of most bacteria including pathogens and most extensively studied in Escherichia coli. E. coli mazEF mediated cell death is a population phenomenon requiring the quorum-sensing (QS) ‘Extracellular Death Factor’ (EDF), the E. coli peptide NNWNN. Penta-peptide NNWNN (EcEDF) enhances the endoribonucleolytic activity of E. coli toxin MazF.The newly discovered extracellular death factor (EDF) is a pentapeptide with the sequence NNWNN in Escherichia coli. It was reported that it participated in the cell death process mediated by toxin-antitoxin system mazEF. Reactive oxygen species (ROS) are recently considered as common factors for bactericidal antibiotics-mediated cell death. Previous study indicated that EDF could scavenge hydroxyl radicals and might act as a signal molecule with dual effects, “death” and “survival”. But the structure-activity relationship of EDF and the effects of EDF on the activity of antibiotics remain unclear. In the present study, our results indicated that tryptophan could be the key residue to the hydroxyl radicals-scavenging activity of EDF, and EDF could protect Escherichia coli from killing by bactericidal antibiotics, but not by DNA-damaging or bacteriostatic antibiotics. Our results could provide novel evidence to understand the role of EDF in drug-resistance.
产品数据
货号 LPMA10233 文献 详细请查看官网 产品名称 Anti Extracellular Death Factor mouse monoclonal antibody 中文名称 Extracellular Death Factor 小鼠单克隆抗体 规格 100ug 适应种属 Escherichia coli 纯化方法 Protein G purified from mice ascites UniProt ID P0AC53 浓度 1mg/ml 宿主 Mouse 克隆性 Monoclonal 亚型 IgG2b 免疫原 NNWNN 分子量 56 kDa 验证应用 Elisa,IHC 验证应用 Liquid 建议稀释比例 Elisa:1:1000~5000,IHC:1:50~100 存储条件 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 °C for frequent use. Store at -20 to -80 °C for twelve months from the date of receipt. 偶联物 Unconjugate 储存溶液 PBS, pH7.4, containing 0.05% proclin300, 50% glycerol. 运输条件 所有可选规格：常温运输或根据产品储存温度要求用蓝冰运输。 注意事项 该产品仅供科研使用
背景介绍
Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.Probable source of extracellular death factor (EDF, sequence Asn-Asn-Trp-Asn-Asn, NNWNN) following processing and amidation. This pentapeptide stimulates cell death mediated by MazF (PubMed:17962566). Artificial peptides with altered sequence show that NNGNN, GNWNG and NWN no longer stimulate MazF's endoribonuclease activity; other peptides (NNGN, GNWMM, NNWNG, NNNWNNN) retain MazF-stimulating activity. NNWNN, NNGN, GNWMM and NNWNG prevent cognate antitoxin MazE from inhibiting MazF; although NNNWNNN stimulates MazF it does not do so in the presence of MazE. EDF also stimulates ChpB's endoribonuclease activity in vitro; in this case NWN partially stimulates ChpB, whereas NNGNN, GNWNN, NNWNG, GNWNG and NNNWNNN do not. Only the wild-type EDF peptide prevents cognate antitoxin ChpS from inhibiting ChpB