Product Highlight

• Configuration-specific monoclonal antibody that recognizes Arf6-GTP, but not Arf6-GDP
• The only available Arf6 activation assay kit directly measures the level of Arf6-GTP
• Providing shorter experimental time
• Could be used to monitor the activation of Arf6 in cells and in tissues by immunohistochemistry

Product Description

ARF6 (ADP-ribosylation factor 6) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a regulatory molecule of phagocytosis.

Currently there is no direct assay to measure the activation of Arf6 GTPases.

NewEast Biosciences Arf6 Activation Assay Kit is based on the configuration-specific monoclonal antibody that specifically recognizes Arf6-GTP, but not Arf6-GDP, and an Arf6 specific rabbit polyclonal antibody. Given the high affinity of monoclonal antibodies to their antigens, the activation assay could be performed in a short time. This assay provides the reliable results with consistent reproducibility.

These anti-Arf6-GTP monoclonal antibodies can also be used to monitor the activation of Arf 6 in cells and in tissues by immunohistochemistry.

NewEast Biosciences Arf6 Activation Assay Kit provides a simple and fast method to monitor the activation of Arf6. Each kit provides sufficient quantities to perform 20 assays.

 

Assay Principle

NewEast Biosciences Arf 6 Activation Assay Kit bases on the configuration-specific anti-Arf 6-GTP monoclonal antibody to measure the active Arf 6-GTP levels, either from cell extracts or from in vitro GTPγS loading Arf 6 activation assays. Briefly, anti-active Arf 6 mouse monoclonal antibody will be incubated with cell lysates containing Arf 6-GTP. The bound active Arf 6 will then be pulled down by protein A/G agarose. The precipitated active Arf 6 will be detected by immunoblot analysis using an anti-Arf 6 rabbit polyclonal antibody, respectively.