DYKDDDDK tag polyclonal antibody
产品名称： DYKDDDDK tag polyclonal antibody
英文名称： DYKDDDDK tag polyclonal antibody
产品编号： PAB10340
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptides of DYKDDDDK tag.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to DYKDDDDK tag.
- Host:
- Rabbit
- Specificity:
- This antibody reacts with FLAG conjugated proteins.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:90000-1:250000)
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5.
Slootstra JW, Kuperus D, Pluckthun A, Meloen RH.Mol Divers. 1997;2(3):156-64.
- 2.
- Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG epitope. Development of an efficient generic protein purification procedure.
Robeva AS, Woodard R, Luthin DR, Taylor HE, Linden J.Biochem Pharmacol. 1996 Feb 23;51(4):545-55.
- 3.
- Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells.
Chubet RG, Brizzard BL.Biotechniques. 1996 Jan;20(1):136-41.
- Applications
- Western Blot (Recombinant protein)
- DYKDDDDK tag polyclonal antibody (Cat # PAB10340) to detect FLAG™ conjugated proteins detects both C terminal linked and N terminal linked FLAG™ tagged recombinant proteins by western blot.
This antibody was used at a dilution of 1 : 2,500 to detect 1.0 ug of recombinant protein containing either the FLAG™ epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein.
A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.
The protein was transferred to nitrocellulose using standard methods.
After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.
LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.