• 经测试应用WB, IHC-Pmore details
• 种属反应性
  与反应: Human
  预测可用于: Mouse, Rat
• 免疫原

  Recombinant fragment, corresponding to amino acids 21-177 of Human Inosine triphosphate pyrophosphatase (BC010138).

• 阳性对照
  • Human fetal liver and heart lysate.

性能

• 形式Lyophilised:Reconstitute in 200ul sterile H2O. Human fetal heart tissue.
• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• 存储溶液pH: 7.20
  Preservative: 0.02% Sodium azide
  Constituents: 1% BSA, 98% PBS
• 浓度由溶解方法决定 
  
• 纯度Immunogen affinity purified
• 克隆多克隆
• 同种型IgG
• 研究领域
  • Signal Transduction
   
  • Metabolism
   
  • Amino Acids

  • Metabolism
   
  • Pathways and Processes
   
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Metabolism
   
  • Types of disease
   
  • Cancer

应用

靶标

• 功能Hydrolyzes ITP and dITP to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. May be the major enzyme responsible for regulating ITP concentration in cells.
• 组织特异性Ubiquitous. Highly expressed in heart, liver, sex glands, thyroid and adrenal gland.
• 疾病相关Defects in ITPA are the cause of inosine triphosphate pyrophosphohydrolase deficiency (ITPA deficiency) [MIM:147520]. It is a common inherited trait characterized by the abnormal accumulation of inosine triphosphate (ITP) in erythrocytes and also leukocytes and fibroblasts. The pathological consequences of ITPA deficiency, if any, are unknown. However, it might have pharmacogenomic implications and be related to increased drug toxicity of purine analog drugs. Three different human populations have been reported with respect to their ITPase activity: high, mean (25% of high) and low activity. The variant Thr-32 is associated with complete loss of enzyme activity, may be by altering the local secondary structure of the protein. Heterozygotes for this polymorphism have 22.5% of the control activity: this is consistent with a dimeric structure of the enzyme.
• 序列相似性Belongs to the HAM1 NTPase family.
• 细胞定位Cytoplasm.

• Anti- Inosine triphosphate pyrophosphatase antibody 图像

  • 

    Western blot - Anti- Inosine triphosphate pyrophosphatase antibody (ab118236)
    All lanes : Anti- Inosine triphosphate pyrophosphatase antibody (ab118236) at 1/1000 dilution
    
    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    
    
    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    
  • 

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti- Inosine triphosphate pyrophosphatase antibody (ab118236)
    ab118236 at 1/100 staining Inosine triphosphate pyrophosphatase in paraffin-embedded Human fetal heart lysate tissue by Immunohistochemistry.