M13 Bacteriophage Coat Protein g8p 小鼠单克隆抗体
产品名称： M13 Bacteriophage Coat Protein g8p 小鼠单克隆抗体
英文名称： M13 Bacteriophage Coat Protein g8p Mouse monoclonal antibody
产品编号： LPMA10066
产品价格： null
产品产地： 中国
品牌商标： Ablepscience
更新时间： null
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产品索引
Capsid protein G8P;Gene 8 protein;M13 procoat;Major coat protein;VIII
产品数据
货号 LPMA10066 文献 详细请查看官网 产品名称 Anti M13 Bacteriophage Coat Protein g8p mouse monoclonal antibody 中文名称 M13 Bacteriophage Coat Protein g8p 小鼠单克隆抗体 规格 100ug 适应种属 Bacteriophage M13 纯化方法 Protein G purified from mice ascites UniProt ID P69541 浓度 1mg/ml 宿主 Mouse 克隆性 Monoclonal 亚型 IgG1 免疫原 M13 Bacteriophage 分子量 8 kDa 验证应用 Elisa,WB,IP,IHC 验证应用 Liquid 建议稀释比例 Elisa:1:1000~5000;WB:1:1000~4000;IP:1:100~200,IHC:1:50~100 存储条件 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 °C for frequent use. Store at -20 to -80 °C for twelve months from the date of receipt. 偶联物 Unconjugate 储存溶液 PBS, pH7.4, containing 0.05% proclin300, 50% glycerol. 运输条件 所有可选规格：常温运输或根据产品储存温度要求用蓝冰运输。 注意事项 该产品仅供科研使用
背景介绍
The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 50 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 2700 copies of p8 to make the coat about 900 nm long.