产品索引
        

        
产品数据 
        

          

            

              

                货号
              
              
LPA28917
              

                文献
              
              

                详细请查看官网 
              
            

            

              

                产品名称
              
              
EYFP mouse Monoclonal Antibody(Mix)
            
            

              

                中文名称
              
              
EYFP 小鼠单克隆抗体(Mix)
            
            

              
规格
              
100ug
              
适应种属
              
Species independent
            
            

              

                纯化方法
              
              
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
              

                UniProt ID
              
              

                
              
            
            

              

                浓度
              
              
1mg/ml
              

                宿主
              
              
Mouse
            
            

              

                克隆性
              
              
Monoclonal
              

                亚型
              
              

            
            

              

                免疫原
              
              
Recombinant Protein of EYFP 
              

                分子量
              
              

            
            

              

                验证应用
              
              
WB
            
            

              

                验证应用
              
              
Liquid
            
            

              

                建议稀释比例
              
              
WB: 1:5000
              

                存储条件
              
              
-20°C/1 year
            
            

              

                偶联物
              
              
Unconjugated 
              

                储存溶液
              
              
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol. 
            
            

              

                运输条件
              
              

                所有可选规格：常温运输或根据产品储存温度要求用蓝冰运输。
              
            
            

              

                注意事项
              
              

                该产品仅供科研使用
              
            
          
        
        

        
背景介绍
        
Enhanced Yellow florescent protein.Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein, derived from Aequorea victoria. Its excitation peak is 514nm and its emission peak is 527nm. Like green fluorescent protein (GFP), it is a useful tool in cell and molecular biology, usually explored using fluorescence microscopy. Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (product of the extinction coefficient and quantum yield). Typically, yellow FPs serve as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is mCFP (monomeric cyan FP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.

        
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