组蛋白H3 K79甲基转移酶抗体
产品名称： 组蛋白H3 K79甲基转移酶抗体
英文名称： KMT4
产品编号： hz-16797R
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
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Rabbit Anti-KMT4 antibody
| 产品编号 | hz-16797R |
| 英文名称 | KMT4 |
| 中文名称 | 组蛋白H3 K79甲基转移酶抗体 |
| 别 名 | Disrupter of telomere silencing protein 1; DOT 1; DOT1; DOT1 like; DOT1 like histone H3 methyltransferase (S. cerevisiae); DOT1 like histone H3 methyltransferase; DOT1 like protein; DOT1, S. cerevisiae, homolog of; DOT1L; EC 2.1.1.43; H3 K79 HMTase; Histone H3 K79 methyltransferase; Histone H3 methyltransferase; Histone H3 methyltransferase DOT1-like; Histone lysine N methyltransferase H3 lysine 79 specific; Histone methyltransferase DOT1L; KIAA1814; DOT1L_HUMAN; KMT4; Lysine N methyltransferase 4. |
| 产品类型 | 甲基化抗体 |
| 研究领域 | 免疫学 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Cow, Sheep, African Green Monkey |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 185kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KMT4:51-150/1739 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011] Function: The KMT4 / Dot1 (disruptor of telomeric silencing) family are a class of histone methyltransferases that play an indirect role in chromatin silencing by targeting a conserved lysine residue in the nucleosome core. Biochemical studies suggest that recombinant Dot1 proteins are active on recombinant nucleosomes, free of any modifications. Subunit: Interacts with MLLT10. Subcellular Location: Nuclear Similarity: Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family. Contains 1 DOT1 domain. SWISS: Q8TEK3 Gene ID: 84444 Database links: Entrez Gene: 84444 Human Entrez Gene: 362831 Rat Omim: 607375 Human SwissProt: Q8TEK3 Human Unigene: 713641 Human Unigene: 228827 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
 Sample:
Liver (Mouse) Lysate at 40 ug Liver (Rat) Lysate at 40 ug Primary: Anti- KMT4 (bs-16797R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 185 kD Observed band size: 170 kD  Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KMT4 Polyclonal Antibody, Unconjugated(bs-16797R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KMT4 Polyclonal Antibody, Unconjugated(bs-16797R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |