phospho-CSEN (Ser63),磷酸化钾通道相互作用蛋白3抗体

产品编号HZ-12167R
英文名称phospho-CSEN (Ser63)
中文名称磷酸化钾通道相互作用蛋白3抗体
别 名CSEN (phospho S63); CSEN (phospho Ser63); p-CSEN (phospho-Ser63); A type potassium channel modulatory protein 3; A-type potassium channel modulatory protein 3; Calsenilin; CSEN; CSEN_HUMAN; DRE antagonist modulator; DRE-antagonist modulator; DREAM; EF hand transcription factor; KCHIP 3; KCHIP3; KCNIP 3; KCNIP3; Kv channel interacting protein 3; Kv channel-interacting protein 3; MGC18289; Potassium channel interacting protein 3; Presenilin binding protein; R74849.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域心血管 神经生物学 通道蛋白 细胞膜受体
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
phospho-CSEN (Ser63),磷酸化钾通道相互作用蛋白3抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量28kDa
细胞定位细胞核 细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human CSEN around the phosphorylation site of Ser63
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-CSEN (Ser63),磷酸化钾通道相互作用蛋白3抗体PubMedPubMed
产品介绍background:
CSEN is a Ca2+-regulated transcriptional repressor that specifically binds to the downstream regulatory elements (DRE). DRE is a regulatory sequence that silences basal transcription and is localized to the promoter region of the gene encoding human prodynorphin, an opioid peptide involved in memory acquisition and pain. DREAM forms functional homotetramers that are required for the interaction with the DRE. This association is highly influenced by calcium, as an increase in Ca2+ directly inhibits DREAM binding and thereby blocks the repressor activity of DREAM. DREAM transcripts are detected in brain, thymus and thyroid gland, and it is expressed as a nuclear protein. DREAM has been shown to inhibit transcription of other proteins containing DRE-like motifs, including the gene encoding for the AP-1 transcription factor c-Fos, suggesting that DREAM may influence a wide variety of cellular genes.

Function:
Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception.
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Probably modulates channels density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and probably KCND3 trafficking to the cell surface. May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation.

Subcellular Location:
Cytoplasm. Cell membrane. Endoplasmic reticulum. Golgi apparatus. Nucleus. Also membrane-bound, associated with the plasma membrane (By similarity). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi.

phospho-CSEN (Ser63),磷酸化钾通道相互作用蛋白3抗体Tissue Specificity:
Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.

Post-translational modifications:
Palmitoylated. Palmitoylation enhances association with the plasma membrane. Proteolytically cleaved by caspase-3.
Phosphorylation at Ser-63 inhibits cleavage by CASP3.

Similarity:
Belongs to the recoverin family.
Contains 4 EF-hand domains.

Gene ID:
30818

Database links:
Entrez Gene: 30818 Human
Entrez Gene: 56461 Mouse
Entrez Gene: 65199 Rat
Omim: 604662 Human
SwissProt: Q9Y2W7 Human
SwissProt: Q9QXT8 Mouse
SwissProt: Q9JM47 Rat
Unigene: 437376 Human
Unigene: 315292 Mouse
Unigene: 74239 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.