DAGLB,二酰基甘油脂肪酶β/DAGLβ 抗体

产品编号HZ-14190R
英文名称DAGLB
中文名称二酰基甘油脂肪酶β/DAGLβ 抗体
别 名Daglb; DGL beta; DGL-beta; DGLB_HUMAN; Diacylglycerol lipase beta; KCCR13L; Sn1 specific diacylglycerol lipase beta; Sn1-specific diacylglycerol lipase beta.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 信号转导 新陈代谢
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep,
DAGLB,二酰基甘油脂肪酶β/DAGLβ 抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量74kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human DAGLB
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
DAGLB,二酰基甘油脂肪酶β/DAGLβ 抗体PubMedPubMed
产品介绍background:
KCCR13L, is a 672 amino acid multi-pass membrane protein that belongs to the AB hydrolase superfamily. DAGL beta uses calcium as a cofactor to catalyze the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), a reaction that is required for axonal growth and for retrograde synaptic signaling at mature synapses. DAGL beta functions at an optimal pH of 7 and its activity is inhibited by p-hydroxy-mercuri-benzoate and HgCl2, but not PMSF. There are three isoforms of DAGL beta that are produced as a result of alternative splicing events.

Function:
Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses.

Subcellular Location:
Cell membrane.

Similarity:
Belongs to the AB hydrolase superfamily. Lipase family.

Gene ID:
221955

DAGLB,二酰基甘油脂肪酶β/DAGLβ 抗体Database links:
Entrez Gene: 221955 Human
Entrez Gene: 231871 Mouse
SwissProt: Q8NCG7 Human
SwissProt: Q91WC9 Mouse
Unigene: 487498 Human
Unigene: 469963 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-DAGLB Polyclonal Antibody, Unconjugated(HZ-14190R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining