Anti-ErbB2 (V777A) mouse monoclonal Antibody-抗体-抗体-生物在线

Anti-ErbB2 (V777A) mouse monoclonal Antibody

商家询价

产品名称： Anti-ErbB2 (V777A) mouse monoclonal Antibody

英文名称： Anti-ErbB2 (V777A) mouse monoclonal Antibody

产品编号： 26080

产品价格： null

产品产地：美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IF、IHC

武汉纽斯特生物技术有限公司

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产品详情

ERBB2 (V777A) [26080]
[100 µL]

Catalog Number: 26080

Gene Symbol: ERBB2/HER2/Neu

Description: Anti-ErbB2 (V777A) mouse monoclonal Antibody

Background: The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. Mutations of ErbB2 protein, including V777A, have been indicated in human cancers.

Immunogen: A synthetic peptide surround the 777A codon of ErbB2 protein.

Tested Applications: ELISA, WB, IF, IHC

Recommended Dilutions:
ELISA: 1:500-1:5000
WB: 1:200-1:2000
IF: 1:100
IHC: 1:50-1:100

Concentration: 0.2 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes V777A mutant but not wild type ErbB2 protein, of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Immunofluorescence:

Immunofluorescence of cells expressing ErbB2 proteins with anti-ErbB2 (V777A) antibody. HEK293T cells were transfected with pCDNA3-GFP- ErbB2 WT plasmid (left column) or pCDNA3-GFP-ErbB2 (V777A) plasmid (right column), then fixed and stained with anti-ErbB2 (V777A) monoclonal antibody (Cat. #26080).