Anti-ErbB2 Rabbit Polyclonal Antibody-抗体-抗体-生物在线

Anti-ErbB2 Rabbit Polyclonal Antibody

商家询价

产品名称： Anti-ErbB2 Rabbit Polyclonal Antibody

英文名称： Anti-ErbB2 Rabbit Polyclonal Antibody

产品编号： 21202

产品价格： null

产品产地：美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IHC

武汉纽斯特生物技术有限公司

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邮箱 : neweast3@126.com

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产品详情

ERBB2 [21202]
[100 µL]

Catalog Number: 21202

Gene Symbol: ERBB2/HER2/Neu

Description: Anti-ErbB2 Rabbit Polyclonal Antibody

Background: The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis.

Immunogen: A synthetic peptide from the central of ErbB2 protein.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:500-1:5000
WB: 1:200-1:2000
IHC: 1:20-1:200

Concentration: 0.2 mg/ml

Host: Rabbit

Format: Liquid

Clonality: Polyclonal

Isotype:

Purity: Purified from serum

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes ErbB2 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles.

Western blot:

Western blot analysis of ErbB2 protein. Purified full length His-tagged ErbB2 protein (Cat. #10208) was blotted with anti-ErbB2 rabbit polyclonal antibody (Cat. #21202).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-ERBB2 polyclonal antibody (Cat. #21202). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4 °C. A HRP-conjugated Goat anti-Rabbit IgG (1:50) was used as secondary antibody.