Anti-MEK1 (D67N) Mouse Monoclonal Antibody-抗体-抗体-生物在线

Anti-MEK1 (D67N) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-MEK1 (D67N) Mouse Monoclonal Antibody

英文名称： Anti-MEK1 (D67N) Mouse Monoclonal Antibody

产品编号： 26224

产品价格： null

产品产地：美国

品牌商标： NewEastBio

更新时间： null

使用范围： ELISA、WB、 IHC

武汉纽斯特生物技术有限公司

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邮箱 : neweast3@126.com

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产品详情

MEK1 (D67N) [26224]
[100ul]

Catalog Number: 26224

Gene Symbol: MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1

Description: Anti-MEK1 (D67N) Mouse Monoclonal Antibody

Background: MEK1 (also known as extracellular signal-regulated kinases, ERKs) plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation and differentiation. Activated upon growth factors stimulation, MEK1 phosphorylates MAPK3/ERK1 and MAPK1/ERK2, thus activates the MAP pathway and regulates transcription. Abnormal of the MEK1 protein, including point mutations, are implicated in diseases such as cardiofaciocutaneous syndrome (CFC) and melanoma.

Immunogen: A synthetic peptide from the internal region of MEK1 which includes the mutation of D67N, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:2000
WB: 1:500-1:1000
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes D67N mutant, but not wild-type MEK1 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant MEK1 (D67N) and wildtype proteins. Purified His-tagged MEK1 (D67N) protein (lane 1) and corresponding wildtype protein (lane 2) were blotted with anti-MEK1 (D67N) monoclonal antibody (Cat. #26224).

Immunofluorescence:

Immunofluorescence of cells expressing MEK1 proteins with anti-MEK1 (D67N) antibody. HEK293T cells were transfected with pCDNA3-GFP-MEK1 (WT) plasmid (left column) or pCDNA3-GFP-MEK1 (D67N) plasmid (right column), then fixed and stained with anti-MEK1 (D67N) monoclonal antibody (Cat. #26224).