Anti-Bag1 Mouse Monoclonal Antibody-抗体-抗体-生物在线
武汉纽斯特生物技术有限公司
Anti-Bag1 Mouse Monoclonal Antibody

Anti-Bag1 Mouse Monoclonal Antibody

商家询价

产品名称： Anti-Bag1 Mouse Monoclonal Antibody

英文名称： Anti-Bag1 Mouse Monoclonal Antibody

产品编号： 26135

产品价格： null

产品产地： 美国

品牌商标： NewEastbio

更新时间： null

使用范围： ELISA, WB, IHC

武汉纽斯特生物技术有限公司
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Bag1 [26135]
[100 µL]		  

Catalog Number:  26135
Gene Symbol:  BAG1, BAG-1, HAP, RAP46
Description:   Anti-Bag1 Mouse Monoclonal Antibody
Background:   BAG family molecular chaperone regulator 1 is a protein that is encoded by the BAG1 gene. It binds to oncogene BCL2, by which enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. BAG gene has been implicated in age related neurodegenerative diseases as Alzheimer's. It has been demonstrated that BAG1 and BAG3 regulate the proteasomal and lysosomal protein elimination pathways, respectively.
Immunogen:   Purified recombinant Bag1 protein, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:500-1:5000 
WB: 1:500-1:2000 
IHC: 1:50-100
Concentration:    1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:   Recognizes Bag1 from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Bag1 protein. Purified full length His-tagged Bag1 protein was blotted with anti-Bag1 mouse monoclonal antibody (Cat. #26135).
Immunohistochemistry:   
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with anti-Bag1 monoclonal antibody (Cat. #26135). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.