Cat code 200225
Species Cross-reactivity H
Key Application WB,IHC,ICC
Antibody type Monoclonal antibody
Purified method Affinity purified
Molecular Weight 38kDa
Sensitivity This antibody detects endogenous levels of PPP1CA and does not cross-react with related proteins.
Immunogen Purified recombinant human PPP1CA protein fragments expressed in E.coli.
UniProt Number P62136
GeneBank ID NM_001008709
Subcellular location Cytoplasm. Nucleus. Nucleus
Formulation Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
Altername serine/threonine-protein phosphatase PP1-alpha catalytic subunit;protein phosphatase 1; catalytic subunit, alpha isoform;serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit;Protein Phosphatase 1 alpha;PPP1CA
Gene_symbol PP-1A; PPP1A; PP1alpha
Summary The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniPort summary Function Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage.
Application Images :
Western blot detection of PPP1CA in MCF7 , K562 ,Hela ,HEPG2 &Jurkat Whole cell lysates using PPP1CA antibody (1:1000 diluted). Predicted band size: 38KDa Observed band size: 38KDa
Immunocytochemistry stain of Hela using PPP1CA antibody (1:300).
IHC of paraffin-embedded human breast cancer using anti- PPP1CA diluted 1/500-1/1000.