Size  100ug
Cat code  200280
Species Cross-reactivity  H
Key Application  WB,ICC,IHC
Host  Mouse
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  100kDa
Sensitivity  This antibody detects endogenous levels of TRIM28 and does not cross-react with related proteins.
Immunogen  Purified recombinant human TRIM28 protein fragments expressed in E.coli.
UniProt Number  Q13263
GeneBank ID  NM_005762
Subcellular location  Nucleus
Formulation  Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername  E3 SUMO-protein ligase TRIM28
EC=6.3.2.-
KRAB-associated protein 1
Short name=KAP-1
KRAB-interacting protein 1
Short name=KRIP-1
Nuclear corepressor KAP-1
RING finger protein 96
Tripartite motif-containing protein 28
Gene_symbol  KAP1; TF1B; RNF96; TIF1B 
Summary  The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
UniPort summary  Function  Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at ´Lys-9´ (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 ´Lys-9 and ´Lys-14´ acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity.
Application Images :  
Western blot detection of TRIM28(C-terminus) in 293T&HepG2 cell lysates using TRIM28(C-terminus) antibody (1:1000 diluted).
Predicted band size: 100KDa
Observed band size: 100KDa.
Immunocytochemistry of HeLa cells using anti- TRIM28 (C-terminus) antibody diluted 1:200.
IHC of paraffin-embedded human Spleen using anti- TRIM28 (C-terminus) diluted 1/500-1/1000.