Cat code 200309
Species Cross-reactivity H
Key Application WB,ICC
Antibody type Monoclonal antibody
Purified method Affinity purified
Molecular Weight 140kDa
Sensitivity This antibody detects endogenous levels TRIM33 and does not cross-react with related proteins.
Immunogen Purified recombinant human TRIM33 protein fragments expressed in E.coli.
UniProt Number Q9UPN9
GeneBank ID NM_015906
Subcellular location Nucleus
Formulation Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername ECTO; PTC7; RFG7; TF1G; TIF1G; FLJ32925; TIFGAMMA; TIF1GAMMA
Gene_symbol ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
Summary The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.
UniPort summary Function Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
Application Images :
Western blot detection of TRIM33(C-terminus) antibody in MCF7&HelaNE cell lysates using TRIM33(C-terminus) antibody (1:1000 diluted). Predicted band size: 120KDa Observed band size: 140KDa
Immunocytochemistry of HeLa cells using anti-TRIM33 (C-terminus) antibody diluted 1:50.
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