来源： HEK293 (人胚胎肾脏细胞株)
纯度： >98% (HPLC及SDS-PAGE测定).
应用： ELISA、Western blotting、细胞培养、动物研究。
Human Adiponectin, Recombinant Protein
Specificity: The amino acid sequence of recombinant human adiponectin is 100% homologous to the amino acid sequence of human serum adiponectin.
Size: 0.1 mg (determined by BCA method)
Source: HEK293 (Human embryonic kidney cell line)
Purity: >98% (HPLC and SDS PAGE analyzed).
Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in PBS buffer
Reconstitution: Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Applications: ELISA, Western blotting, cell culture and/or animal studies
Stability/Shelf Life: The lyophilized protein remains stable until the expiry date when stored at -20°C.
Quality Control Test: BCA - to determine content of the protein
SDS PAGE - to determine purity of the protein
Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue. In the circulation, adiponectin is present as three different oligomeric complexes, including the high molecular weight (HMW), the middle molecular weight (MMW, also called hexamer) and low molecular weigh (LMW, also called trimer) forms. Different oligomeric complex of adiponectin activates different signaling pathways and exerts distinct functions.
Full-length adiponectin was expressed in HEK293 cells stably transfected with pchAd-F vector encoding FLAG epitope-tagged human adiponectin. Recombinant adiponectin was purified using anti-FLAG M2 affinity gel (Sigma-Aldrich) and was eluted with FLAG peptide. FLAG peptides were removed by an extensive wash with an excessive volume of saline. Highly sensitive matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass-spectrometric analysis confirmed that FLAG peptide was not detectable in the protein solution.
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•Yamauchi T, Kamon J, Minokoshi Y, Ito Y, Waki H, Uchida S, Yamashita S, Noda M, Kita S, Ueki K, Eto K, Akanuma Y, Froguel P, Foufelle F, Ferre P, Carling D, Kimura S, Nagai R, Kahn BB, Kadowaki T. Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMPactivated protein kinase. Nat Med. 2002; 8:1288-1295.
•Berg AH, Combs TP, Du X, Brownlee M, Scherer PE. The adipocyte-secreted protein Acrp30 enhances hepatic insulin action. Nat Med. 2001; 7:947-953.