重组小鼠脂联素球状结构域上海美吉生物医药科技有限公司

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产品名称: 重组小鼠脂联素球状结构域
英文名称: Mouse Adiponectin Globular, Recombinant Protein
抗体货号: RD001-04
产品规格: 0.1mg (BCA法测定)
产品产地: BIOVENDOR
品牌商标: BIOVENDOR
价    格: 4200元
抗原来源: 大肠杆菌(E. coli)
抗体来源: 
适用物种: 
应用范围: 
更新时间: 2019/6/29 12:15:00
详细资料:  实验方法技术资料
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了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
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上海美吉生物医药科技有限公司
地址:
上海市浦东新区国际医学园区康新公路3399弄3号楼
邮编:
201203
电话:
021-51875086
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021-51875086-8002
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产品详细描述

货号:BW001-04
 
氨基酸序列:MSWWHHHHHH NWNIPTTQDT TQDLWFEGAM GGEA AYVYRSAFSV GLETRVTVPN VPIRFTKIFY NQQNHYDGST GKFYCNIPGL YYFSYHITVY MKDVKVSLFK KDKAVLFTYD QYQEKNVDQA SGSVLLHLEV GDQVWLQVYG DGDHNGLYAD NVNDSTFTGF LLYHDTN
 
共171个氨基酸残基,其中N末端的31个氨基酸残基(下划线)为His-tag及酶切位点。其余部分与小鼠全长脂联素的108-248位的氨基酸序列完全相同。
 
规格:0.1mg (BCA法测定)
来源: 大肠杆菌(E. coli)
 
产品形态:冻干粉(无菌过滤,0.5mg/ml脂联素,PBS缓冲液)
 
溶解:加0.2ml去离子水,室温下彻底溶解,最终浓度为0.5mg/ml。
 
保存条件: -20℃保存。建议溶解后分装保存,避免反复冻融。溶解后可以暂时保存在4℃至两周。
 
应用: ELISA、Western blotting、细胞培养、动物研究。
 
有效期:见包装。
 
质控检测:BCA-测定蛋白浓度
SDS PAGE-测定蛋白纯度
 
注意:本产品仅用于科研。

Mouse Adiponectin Globular, Recombinant Protein

AA sequence: MSWWHHHHHH NWNIPTTQDT TQDLWFEGAM GGEA AYVYRSAFSV GLETRVTVPN VPIRFTKIFY NQQNHYDGST GKFYCNIPGL YYFSYHITVY MKDVKVSLFK KDKAVLFTYD QYQEKNVDQA SGSVLLHLEV GDQVWLQVYG DGDHNGLYAD NVNDSTFTGF LLYHDTN
Total 171AA. N-terminal underlined amino acids are His-tag and the protease cleavage site (31AA). The AA sequence is homologous to the 108-248 amino acid sequence of the mouse full length adiponectin.
Size: 0.1 mg (determined by BCA method)
Source: E. coli
Formulation: Sterile filtered and lyophilized from 0.5 mg/ml in PBS buffer
Reconstitution: Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Applications: ELISA, Western blotting, cell culture and/or animal studies
Stability/Shelf Life: The lyophilized protein remains stable until the expiry date when stored at -20°C.
Quality Control Test: BCA - to determine content of the protein
SDS PAGE - to determine purity of the protein
Note: For research use only

Introduction:
Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue. In the circulation, adiponectin is present as three different oligomeric complexes, including the high molecular weight (HMW), the middle molecular weight (MMW, also called hexamer) and low molecular weigh (LMW, also called trimer) forms. Different oligomeric complex of adiponectin activates different signaling pathways and exerts distinct functions.

• Xu A, Wang Y, Keshaw H, Xu LY, Lam KS, Cooper GJ. The fat-derived hormone adiponectin alleviates alcoholic and nonalcoholic fatty liver diseases in mice. J Clin Invest. 2003; 112:91-100.
• Pajvani UB, Du X, Combs TP, Berg AH, Rajala MW, Schulthess T, Engel J, Brownlee M, Scherer PE. Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications fpr metabolic regulation and bioactivity. J Biol Chem. 2003; 278:9073-85.
• Wang Y, Xu A, Knight C, Xu LY, Cooper GJ. Hydroxylation and glycosylation of the four conserved lysine residues in the collagenous domain of adiponectin. Potential role in the modulation of its insulin-sensitizing activity. J Biol Chem. 2002; 277:19521-19529.
• Yamauchi T, Kamon J, Minokoshi Y, Ito Y, Waki H, Uchida S, Yamashita S, Noda M, Kita S, Ueki K, Eto K, Akanuma Y, Froguel P, Foufelle F, Ferre P, Carling D, Kimura S, Nagai R, Kahn BB, Kadowaki T. Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMPactivated protein kinase. Nat Med. 2002; 8:1288-1295.
• Berg AH, Combs TP, Du X, Brownlee M, Scherer PE. The adipocyte-secreted protein Acrp30 enhances hepatic insulin action. Nat Med. 2001; 7:947-953.

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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