Cat code 200143
Species Cross-reactivity H,M
Key Application WB,ICC
Antibody type Monoclonal antibody
Purified method Affinity purified
Molecular Weight 38kDa
Sensitivity This antibody detects endogenous levels of NPM1 and does not cross-react with related proteins.
Immunogen Purified recombinant full length of human NPM1 protein expressed in E.coli.
UniProt Number P06748
GeneBank ID NM_002520
Subcellular location Nucleus, Cytoplasm
Formulation Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername B23; NPM; MGC104254
Gene_symbol B23; NPM
Summary This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
UniPort summary Function Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules.
Application Images :
Western blot detection of NPM1 antibody in Hela,Jurkat&3T3 cell lysates using NPM1 antibody (1:1000 diluted). Predicted band size:33KDa. Observed band size:38KDa.
Immunocytochemistry of HeLa cells using anti- NPM1 antibody diluted 1:200